Stabilization of Structure of Monomeric and Multimeric Proteins – Role of Cosolvents(Timasheff & Arakawa 1989). In the presence of cosolvents, the two state equilibrium between the native and denatured states of a protein is shifted towards the native or stable form of the protein (Timasheff&...
Surface Ligand‐Density Switches Glycovesicles Between Monomeric and Multimeric Lectin RecognitionsCarbohydratesCell surface mimicsLectinsPolydiacetylene polymersvesiclesCarbohydrate-protein interactions define a multitude of cellular recognition events. We present herein synthetic glycovesicles as cell-surface mimics ...
In summary, the succession of steps are as follows: Multimeric protein complexes, called the exocyst, tether vesicles to a target membrane, followed by interaction with Rab-GTPases and formation of a t-(Q-)/v-(R-) SNARE trans-complex which mediates vesicle docking for subsequent fusion (...
Recent biophysical studies indicate that receptors which, according to their migration in SDS–PAGE, were previously thought to be monomers, form dimeric or multimeric structures in the absence of their ligand. This is the case for the bacterial chemotaxis receptor (29, 24), the EPO-R (53, 30...
Mechanistically, this is very interesting and shows that it is possible to regulate monomeric proteases in a manner similar to multimeric proteins and receptors. Our work also shows that partial inhibition arises from a structural change in the catalytic site, just as partial agonism or antagonism ...
For instance, two separate binding sites for pCRP were located on domains SCR4–6 and SCR16–20, respectively36. On the other hand, FH showed strong binding to denatured, monomeric CRP, rather than to the native multimeric vfoarrmian37t,3389.–4O1.fApsaraticcounlsaerqnuoetne,ceth, ein...
Human VEGF165 is also a soluble multimeric protein, being a dimer that comprises two identical polypeptides. The approved anti-VEGF antibody drug bevacizumab has the potential to aggregate the dimeric VEGF protein, as illustrated by the crystal structure of the VEGF-bevacizumab Fab complex (Structure...
We have developed a range of computationalde novoprotein design methods capable of tackling several important areas of protein design. These include the design of monomeric proteins for increased stability, protein-peptide complexes and multimeric proteins for increased binding affinity and aggregation ...
Fusion and protein with the immunoglobulin fold structure, a protein that can serve as a sub-unit structure A process for preparing a multimeric protein consisting of monomeric protein were to (A) the a monomeric protein, preparing a protein in the form of insoluble granules in the cells of ...
A monomeric protein delivery device is also provided, comprising a supply of a multimeric protein 4, complex, comprising multimeric protein and an aggregating agent; a flow path having a subcutaneous exit 8, fluidly coupling the multimeric protein supply to the exit; a porous surface assembly ...