Three protocols for monocyte isolation from peripheral blood mononuclear cells (PBMCs) were evaluated: three-hour incubation on FBS-coated flasks; an overnight incubation on FBS-coated flasks; and Magnetic Activated Cell Sorting (MACS). Additionally, five different concentrations of human recombinant ...
PBMCs can be isolated from human blood or peripheral leukopacs by density gradient centrifugation. Monocytes can be isolated from PBMCs using Invitrogen Dynabeads Untouched Human Monocytes Kit, which isolates pure and viable monocytes from PBMC by negative isolation. The kit ...
Figure 1.Typical EasySep™ Human Monocyte Isolation Kit (Catalog #19359) Starting with PBMCs prepared from human peripheral blood, the monocyte cell content (CD14+CD16-) of the isolated fraction obtained without (middle plots) or with (bottom plots) EasySep™ Human Platelet Removal Cocktail is...
PBMC isolation PBMCs of BC patients were isolated from heparin-ficoll containing CPT sample tubes (no. 362780, Becton-Dickinson). Within 1 h of blood withdrawal, CPT blood tubes were centrifuged for 20 min at 1650 x g and RT to separate PBMCs by an inert gel barrier. After sample transpo...
Monocytes were isolated from PBMC by negative isolation using the Pan Monocyte Isolation Kit (Miltenyi Biotec) or the EasySep monocyte isolation kit (Stem Cell Technologies, Cologne, Germany) following the instructions of the companies. Negatively isolated monocytes contained > 92% CD14+ monocytes...
The PBMCs were then used in VOAs to determine the appropriate conditions for QVOA development. All VOAs were completed on MDMs derived from fresh never-frozen PBMCs or negatively isolated monocytes (Pan Monocyte isolation kit, human; Miltenyi Biotec). PBMCs or isolated monocytes were plated at ...
We interrogated the expression of the potentially causal gene (TMEM187) in peripheral blood mononuclear cells (PBMCs) from celiac patients at onset, on a gluten-free diet, potential celiac patients and non-celiac controls. Results The XWAS and gene-based analyses identified 13 SNPs and 25 ...
For even faster cell isolations, we recommend the new EasySep™ Human Monocyte Isolation Kit (19359) which isolates cells in as little as 12.5 minutes. Advantages:• Fast, easy-to-use and column-free • Up to 95% purity • Untouched, viable cells ...
Cell isolation We collected 2 ml peripheral blood each time from the COVID-19 patients. PBMCs were freshly isolated from whole blood by using a density gradient centrifugation with Ficoll-Paque and cryopreserved for the subsequent generation of a single-cell RNA library. ...
Methods Monocyte subsets isolation. Peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll- Hypaque density centrifugation from apheresis cones, buffy coats or whole blood donated by healthy adult donors. Apheresis cones and buffy coats were provided by the Blood Donation Centre, Health...