The IgG1 model monoclonal antibody (mAb) was provided by Sandoz Biopharmaceuticals, Schaftenau, Austria. It was produced in CHO cells and 55 mg/ml of the drug substance were formulated in a 50 mM sodium phosphate buffer at pH 6.2. The mAb had an extinction coefficient of 1.7 ml g−1 ...
Second generation: chimeric antibodies (-ximab) with 33% mouse protein that contained variable domains of murine mAbs and constant domain of human antibody and showed lower immunogenicity. Third generation: humanized antibody (-zumab) generated by techniques, such as CDR (complementarity determining ...