amonoclonalantibody-basedcompetitiveelisaforthedeterminationofruscogenin XuYu,eta1./ChinJNatMed,2014,12(10):794-799specificitytoasingleepitope,theirhomogeneousstructure,andtheirabilitytobemass—producedD2J.Inthisreport.themonoclonalantibodyagainstRUSispreparedanddemon—stratedtobeaveryusefultooltoforthequalitycontrolor...
FIG. 11(A)-11(B): Epitope mapping and specificity of representative monoclonal antibodies raised against recombinant human TN. 11(A) Dot blotting analysis of polyclonal antibodies (“pAb”) and monoclonal antibodies (“mAbs”) using synthetic peptides corresponding to sequences shown in FIG. 6B....
An inELISA and an icELISA were used to detect the antibody titers and IC50 values of ZEN in the supernatants at different passages to determine the stability of the hybridoma cells secreting an antibody. 4.7.2. Identification of Affinity, Sensitivity, and Specificity of ZEN mAbs The Batty ...
Characterizing the properties of an antibody typically includes determining its sensitivity, specificity, and any additional properties that might be relevant for its intended use. The 9G9 antibody type was IgG1. Based on this, the ic-ELISA (the IC50 and LOD were 0.19 ng/mL and 0.04 ng/mL,...
MN showed the lowest specificity at 79.2%, indicative of the significant number of false positives detected, perhaps due to cross-reactivity. Although pvNT has the lowest detection threshold based on the ROC curve (label not visible), it is accepted that pvNT will unlikely reach 100% ...
toxins Article A Monoclonal–Monoclonal Antibody Based Capture ELISA for Abrin Christina C. Tam 1, Luisa W. Cheng 1, Xiaohua He 1, Paul Merrill 1, David Hodge 2 and Larry H. Stanker 1,* 1 Foodborne Toxin Detection and Prevention Research Unit, Western Regional Research Center, Agricultural ...
Evidently, further studies are needed into IHC applications of this antibody in terms of specificity and limitations. In addition, some new antibodies with equivalent or better performance would be valuable. Recently, a rabbit monoclonal antibody, MRQ-67, was generated using B-cell cloning technology...
Mouse macrophage hemagglutinin (sheep erythrocyte receptor) with specificity for sialylated glycoconjugates characterized by a monoclonal antibody. J. Exp. Med. 1989, 169, 1333–1346. [CrossRef] [PubMed] 47. Delputte, P.L.; Van Breedam, W.; Delrue, I.; Oetke, C.; Crocker, P.R.; ...
Keywords: Bacillus anthracis; anthrax; monoclonal antibody; phage display; epitope; lethal factor; protective antigen; mass spectrometry 1. Introduction Anthrax is caused by infection with Bacillus anthracis, a Gram-positive, spore-forming, bacterium of the Bacillus cereus group. B. anthracis is a ...
Moreover, the specificity of M-Ig should be defined and correlated with clinical data. Other complementary immunological tests can be of help in the final diagno- sis of some specific entities, such as complement studies in type II cryoglobulinemia and xanthomatosis. The pathophysiological mechanism ...