13; and a light chain polypeptide comprising contiguous amino acid sequences of a CDRL1, a CDRL2; and a CDRL3 of a variable light chain polypeptide having the amino acid sequence of SEQ ID NO: 17, wherein the antibody, or antigen binding fragment thereof binds to protective antigen (PA)...
Protein A/G Purification Column Staining Solution for Cells and Tissue Positive Control for Antibody Tissue/Sections Customized Service Phosphorylated Antibody Customized Service Western Blot (WB) Experiment Service Immunohistochemistry (IHC) Experiment Service ...
The C-terminal lysine of the Fc region may be removed, for example, during production or purification of the antibody, or by recombinantly engineering the nucleic acid encoding a heavy chain of the antibody. Accordingly, an intact antibody as used herein may be an antibody with or without ...
toxins Article A Monoclonal–Monoclonal Antibody Based Capture ELISA for Abrin Christina C. Tam 1, Luisa W. Cheng 1, Xiaohua He 1, Paul Merrill 1, David Hodge 2 and Larry H. Stanker 1,* 1 Foodborne Toxin Detection and Prevention Research Unit, Western Regional Research Center, Agricultural ...
ZEN mAb Production and Purification The in vivo-induced ascites method was used to produce several ZEN mAbs [50]. Briefly, a mature female Balb/c mouse was intraperitoneally injected with 0.8 mL of paraffin ten days before receiving an intraperitoneal injection of the positive hybridoma cells (1...
PCR amplification products were verified by 2% agarose gel electrophoresis and purified using the GeneJET PCR Purification Kit (Cat. No. K0701, Thermo Fisher Scientific). Sanger DNA sequencing was performed at Genewiz (https: //www.genewiz.com; accessed on 2 July 2018) using the same forward ...
Purification of the mAbs from the hybridoma supernatant was performed on a protein G affinity column using a peristaltic pump. Isotyping of the different anti-hSn and anti-mSn mAbs was performed using a mouse Immunoglobulin Isotyping ELISA Kit and rat isotyping kit from BD Biosciences respectively....