Broad-spectrum monoclonal antibodies (mAb) that can bind multiple serotypes of enteroviruses have gradually become a research hotspot in the diagnosis, prevention and treatment of HFMD. Methods In this study, a mAb 1H4 was obtained using monoclonal antibody technology by immunizing purified virus ...
Adeno-associated virus mediated expression of monoclonal antibody MR191 protects mice against Marburg virus and provides long-term expression in sheep. Gene Ther. 2022; https://doi.org/10.1038/s41434-022-00361-2. Hinderer C, Bell P, Louboutin JP, Zhu Y, Yu H, Lin G, et al. Neonatal ...
Human venous blood was drawn and anticoagulated with EDTA. Hematologic parameters were determined by standard laboratory procedures.3 Gene mapping of the ζ-α-globin gene cluster was performed by Southern blot analysis as previously described.4 SBIA for ζ-globin chains. The procedures for the assa...
Monoclonal antibodies were the first successful targeted anti-cancer therapeutics in the modern era. In the 1970s elucidation of hybridoma and molecular biology technologies established mechanisms to produce and modify monoclonal antibodies with therapeu
High quality, well-validated antibodies are needed to mitigate irreproducibility and clarify conflicting data in science. We describe an epitope-directed monoclonal antibody (mAb) production method that addresses issues of antibody quality, validation an
Bray GL, Thompson AR. Partial factor IX protein in a pedigree with haemophilia B due to partial gene deletion. J Clin Invest 1986;77:1194–200. Smith KJ. Monoclonal antibodies to coagulation factor IX define a high frequency polymorphism by immunoassays. Am J Hum Genet 1985;37:668–79. ...
Integrative evaluations, including gene expression and phosphokinome profiling in tumour samples and liquid biopsies, could represent suitable tools for dose-finding clinical trials when preclinical studies have established clear correlations between a molecular signature and drug efficacy. In addition, we ...
in the H chain CDR3; and 5) the VH gene segment of one anti-DNA antibody (D42) was found to be very similar to the VH gene segment of a CBA mouse hybridoma antibody (6G6) which binds to the environmental Ag phosphocholine... D Eilat,DM Webster,AR Rees - 《Journal of Immunology》...
Methods: The atypical cadherin FAT1 was discovered as a novel CRC-associated protein by using a monoclonal antibody (mAb198.3). FAT1 expression was assessed in CRC cells by immunohistochemistry (IHC), immunoblots, flow cytometry and confocal microscopy. In addition, in vitro and in vivo tumour ...
gene expressionidentificationmonoclonal antibodiesnecrosispathogensPotato virus Y (PVY) is an important viral pathogen of potato responsible for reducing tuber yield and quality across the globe. The PVY N and PVY NTN strains, the latter of which induces potato tuber necrotic ringspot disease (PTNRD)...