Colorectal cancer (CRC) is the third most common cancer and the second leading cause of cancer deaths worldwide. Besides common therapeutic approaches, such as surgery, chemotherapy, and radiotherapy, novel therapeutic approaches, including immunotherapy
To address specificity, the staining profile of 4B12 has been compared to a polyclonal antibody generated against a CCR7 peptide (Bjorkdahl et al). This analysis confirms that the polyclonal antibody and 4B12 stain similar populations of cells. Furthermore, 4B12 stains mouse CC...
JC and JAH participated in the ELISAs for monoclonal and polyclonal antibodies, as well as protein determination and the collection of data for the present manuscript. JC and MEP, together with PL performed dot-blot assays, RIAs, tissue extraction, and preparation of peptide fractions from brain...
et al. Resistance of SARS-CoV-2 variants to neutralization by monoclonal and serum-derived polyclonal antibodies. Nat. Med. 27, 717–726 (2021). Article CAS PubMed PubMed Central Google Scholar Garcia-Beltran, W. F. et al. Multiple SARS-CoV-2 variants escape neutralization by vaccine-...
The rabbit has long been used as a source of polyclonal antibodies. Not only are they able to develop antibodies that recognize diverse epitopes (including post-translational modifications such as glycosylation26 and phosphorylations), rabbits are evolutionarily distant from mice and may recognize epitop...
(J. Immunol. Methods 87, 79, 1986) describe the production of polyclonal anti-hirudin antibodies (antisera) by immunization of sheep with hirudin. The article expressly notes the limited success of the immunization procedure and the difficulties in producing antisera at all. The same research ...
Herein, we report the generation of humanized monoclonal antibodies blocking FABP4 activity for breast cancer treatment in mouse models. One clone, named 12G2, which significantly reduced circulating levels of FABP4 and inhibited mammary tumor growth, was selected for further characterization. After co...
Thus, this new capture ELISA uses mAbs for both capture and detection; has no cross-reactivity against other plant RIPs; and has a sensitivity comparable to other reported capture ELISAs using polyclonal antibodies as either capture or detector. ...
(Fig.1c), signifying that the polyclonal response incorporated cross-reactive antibodies. The prime-boost OMV regimen induced robust IgG1, IgG2, IgG3, and IgG4 subclass responses, as well as IgM (Fig.1d). Therefore, immunising Kymouse with an OMV cocktail induced a human antibody response ...
Fig. 2: Polyclonal antibodies to PyTRAP inhibit parasite invasion, traversal, and in vivo infection. Mice were immunized three times with PyTRAP or PyCSP ectodomains. a Immune sera were used to verify binding to Py sporozoites via immunofluorescence. Shown is a representative example of fixed, ...