And why do different cells have different transcription rates? Is it really reasonable to assume, as we have here, that these rates are ‘frozen’, and remain as they are for all time in a given cell? Interpretable and tractable modeling framework for transcription rate variation We propose a...
The human gut microbiome is a complex microbial community that is strongly linked to both host health and disease. However, the detailed molecular mechanisms underlying the effects of these microorganisms on host biology remain largely uncharacterized. T
Upregulation of the antiapoptotic BCL family members is an important mechanism through which cancer cells avoid cell death in the face of pathway dysregulation, radiation, and chemotherapy. The structure of BCL-xL bound to BAK peptide (16-mer, Kd = 340 nM) suggested that this PPI might be ...
In the current study, (1) rat cortical neurons (RCNs) were used as the cellular basis for investigation and (2) were either seeded with hAD tau or left as unseeded unseeded. Cells were (3) treated with 8 μm Anle138b for three different durations then (4) RNA-Seq experiments were ...
nerve growth factor (NGF), basic fibroblast growth factor (bFGF), or brain-derived neurotrophic factor[34]. Amniotic fluid-derived stem cells (AFSCs) have been used as a stem cell source for tissue engineering applications[35,36]. Our group has demonstrated that AFSCs have important and uniqu...
The modulation of ESM-1 under hypoxia was investigated, and it was confirmed that ESM-1 expression was induced by HIF1-伪 and significantly attenuated by small interfering RNA (siRNA) targeting HIF-1伪 in CRC cells. These results showed that ESM-1 is significantly overexpressed, which is ...
Knockdown of RGMb, in HECV cells, had minimal effects on tubule formation, brought about a general, although non-significant increase in cell growth and enhanced cell migration. Similarly, no significant effect of RGMb knockdown was found in vivo using a co-inoculation angiogenesis model. ...
μl of dig-wash buffer, and cells were incubated at room temperature for 1 h. cells were washed using the magnet stand twice for 5 min in 1 ml dig-wash buffer to remove unbound antibodies. 0.04 μm of pa-tn5 was prepared in 150 μl dig-med buffer (0.05% digitonin, 20...
1b and Supplementary Fig. 1a). To determine whether the block in filamentation was due to compromised cell viability, C. albicans cells grown in Lactobacillus-conditioned medium were incubated with propidium iodide, a red-fluorescent DNA-binding dye only permeable to dead cells. Lactobacillus-...
and allowing interpretation of metabolic changes at the systems-level in health and disease, from whole organisms to single cells.177,178,179,180,181,182,183,184,185,186,187,188,189,190Metabolomics mass spectrometry-based can rapidly discover small molecule metabolites and improve the understanding...