研究开展了大规模肿瘤细胞样本的蛋白质组学检测,全景描绘了KRAS突变肿瘤的蛋白质组和磷酸化信号通路,实现了基于多组学的KRAS突变肿瘤的分子分型,并在此基础上揭示了KRAS突变肿瘤的精准治疗新策略。 蛋白组学和磷酸化蛋白组学鉴定结果 作者对43种KRAS突变和10种KRAS野生型(KRAS WT)的癌细胞系进行了蛋白组学和磷酸化蛋...
The lowest (most negative) binding free energy obtained in the WT鈥揹elafloxacin complex indicated a better affinity and more stable interaction than the mutant complexes. The key residues of GyrA involved in interaction with delafloxacin were identified using their contribution to binding energy ...
Major depressive disorder (MDD) is a psychiatric disease of still poorly understood molecular etiology. Extensive studies at different molecular levels point to a high complexity of numerous interrelated pathways as the underpinnings of depression. Major systems under consideration include monoamines, stress...
As a result, Th2 cells largely accumulated in the lungs of HDMLPS-sensitized Il6−/− compared to those of WT mice (Fig. 1B–D). These data show that IL-6 is required to prevent the accumulation of allergen-induced Th2 cells. In agreement, while sensitization with HDMLPS prevented...
atamab (FGFR inhibitor) plus pembrolizumab had an encouraging effect in FGFRWTmetastatic urothelial carcinoma [237]. c-MET is also known as hepatocyte growth factor receptor (HGFR). Activated c-MET signaling triggers downstream MAPK, PI3K-AKT, RAC1, and FAK pathways [238]. c-MET signaling ...
Molecular dynamics simulations of sulfuric acid solutions at room temperature and at concentrations up to 40 wt% have been performed. The dissociation of the acid in water has been implicitly taken into account by assuming that the simulated systems are made up of the following molecular constitue...
At approximately 4 weeks of age, the spleens and lymph nodes of the triple knockout mice were substantially larger compared to wild type (WT) mice due to hyperproliferation of constitutively active B and T lymphocytes. The triple knockout mice also developed autoimmune diseases such as ...
Crystals were grown by hanging drop vapor diffusion in drops containing 2 μL of the protein solution mixed with 2 μL of 0.2–0.3 M magnesium acetate or calcium acetate, 20–30% (wt/vol) PEG 3350 equilibrated against 900 μL of the same solution. Crystals were transferred to 35% (wt/...
Supplementary Table 1 tabulates the surface compositions of these polyamide layers. The S atom can hardly be detected in the pristine polyamide layer although there is a tiny amount of sodium dodecyl sulfate (SDS; 0.2 wt%) in the MPD aqueous solution. By incorporating STCAss and SCA, the...
Bars represent the difference in the calculated potency (pIC50) of the inverse agonist for mutations relative to the WT ghrelin receptor. Data are colored according to extent of effect. nd not detected, **P < 0.01, ***P < 0.001 (one-way analysis of variance (ANOVA) followed by ...