Tris Base Sigma-Aldrich Cat#252859 Tris HCl Sigma-Aldrich Cat#T3253 NaH2PO4 Sigma-Aldrich Cat#S0751 Na2HPO4 Sigma-Aldrich Cat#S9763 Sytox green Invitrogen Cat#S7020 BlottoA Santa Cruz Biotechnology Cat#Sc2333 Normal goat serum (NGS) Vector Laboratories Cat#S-1000 Bovine serum albumin (BSA) Si...
Until now, the contribution of BK channels to the behavioral effects of ethanol has been studied by genetically manipulating the channel-forming α subunit in worms and flies, and the auxiliary β subunits in mice. Studies in invertebrates showed that BK α mediates the intoxicating effects of eth...
After completion of the IEF, proteins on the strip were equilibrated for 15 min in a buffer containing 6 M urea, 1% DTT, 30% glycerol, 50 mM Tris base, 2% sodium dodecyl sulphate (SDS), and DTT and then for an additional 15 min in the same solution except that DTT was replaced by...
We find that circRNA expression pattern is tremendously changed during GBC development. Among dozens of significantly changed circRNAs, a circRNA generated from the oncogene ERBB2, named as circERBB2, is one of the most significant changes. CircERBB2 promotes GBC proliferation, in vitro and in viv...
The final mitochondrial pellet was lysed with 2% CHAPS (Bio Basic, Markham, Canada) in Tris-buffered saline (TBS; Biosesang; Seongnam-si, Gyeonggi-do, Republic of Korea). The lysate was measured using a BCA assay kit (Thermo Fisher Scientific). Transfection with Cox5a siRNA, miRs, and ...
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TBE 10X Buffer (1L)(Available in a 10X solution from Promega [Cat.# V4251]):Dissolve 108g of Tris base and 55g boric acid in 900ml deionized water. Add 40ml 0.5M EDTA (pH 8.0) and increase the final volume to 1L. Store at room temperature or 4°C. ...
We also use optional cookies for advertising, personalisation of content, usage analysis, and social media. By accepting optional cookies, you consent to the processing of your personal data - including transfers to third parties. Some third parties are outside of the European Economic Area, with...
After incubation with the appropriate horseradish peroxidase-coupled secondary antibodies (Dako), the proteins were visualized using homemade-enhanced chemiluminescence technology (1:1 mixture of solution A (100 mM Tris, 2.5 mM luminol, 0.4 mM coumaric acid) and solution B (100 mM Tris,...
The oligos were dissolved in a buffer containing 0.2 M NaCl, 0.1 mM EDTA, and 10 mM Tris HCl pH 7.5 and mixed to have 10 μM of each in a PCR tube. After heating at 95 °C for 3 min, the oligo pairs were annealed by gradual cooling from 94 °C to 25 ...