Additional blood samples from the caudal sinus were collected from the West Bengal (WB) populations and preserved in EDTA-Tris buffer; these specimens were subsequently released after taking necessary scale counts. Our study is reported in accordance with the ARRIVE 2.0 guidelines (Animal Research: R...
2 mM Mg2+. The reaction was started by adding 100 μL of the Amplex Red reaction mixture (100 mM Tris pH 7.4, 10 mM MgCl2, 2 U/mL horseradish peroxidase, 0.2 U/mL choline oxidase, 8 U/mL alkaline phosphatase, 0.5 mM sphingomyelin, 0.2% (w/v) Triton X-100...
Exon 1 was amplified similarly with the addition of 10% dimethyl sulfoxide and exon 13 was amplified in 200 μmol/L each dNTP, 3 mmol/L MgCl2, 50 mmol/L KCl, 0.1% Triton X-100 and 10 mmol/L Tris-HCl pH 8.8. In the cases of exons 2-15, PCRs included an initial denaturing step...
Synthesis and characterization of an unprecedented water-soluble tris-functionalized Anderson-type polyoxometalate Yunshen Zhang, Hongli Jia, Qi Li, Yichao Huang, Yongge Wei Article 128555 Article preview Sorry, this component is empty. select article Synthesis and structural study of some <em>N</em...
while the LNP/DNA complexes were tested in experimental wells, each containing 1 μg of DNA. All samples were loaded onto a gel containing 1% agarose and run in l × Tris–EDTA buffer (pH 8.3) using a gel electrophoresis system (Bio-Rad, Hercules, CA, USA). The gel was then vis...
amination on a low molecular weight heparin, in the presence of an ammonium halide salt and a borohydride salt, at a temperature of between 50 and 80° C., andb) then performing an acylation with a group —(R1)i-Biot in activated ester form, in the presence of a base in aqueous ...
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The oligos were dissolved in a buffer containing 0.2 M NaCl, 0.1 mM EDTA, and 10 mM Tris HCl pH 7.5 and mixed to have 10 μM of each in a PCR tube. After heating at 95 °C for 3 min, the oligo pairs were annealed by gradual cooling from 94 °C to 25 ...
The formylpeptide receptors (FPRs) mediate pattern recognition of formylated peptides derived from invading pathogens or mitochondria from dead host cells. They can also sense other structurally distinct native peptides and even lipid mediators to either
After completion of the IEF, proteins on the strip were equilibrated for 15 min in a buffer containing 6 M urea, 1% DTT, 30% glycerol, 50 mM Tris base, 2% sodium dodecyl sulphate (SDS), and DTT and then for an additional 15 min in the same solution except that DTT was replaced by...