Of note, the line width of an NMR signal is related to the molecular weight of the protein tumbling in solution. Thus, the line broadening observed during complex formation will reflect the increase in the molecular weight of the complex. In addition, the stability of the complex, that is,...
Imidazole catalysis of high molecular weight anhydride esterificationdoi:US3361776 AVries Louis De
1H NMR, FT-IR and comparative tests indicated that the electrostatic interaction between imidazole cation and TFSI- anion and intermolecular hydrogen bonding between three compounds also contribute to the formation of supramolecular ionogel. Ionogels with different gel-sol phase transition temperatures can ...
The result of sedimentation velocity analysis in 50mm imidazole-HCl buffer at pH 7.0 showed that human -casein was present in a monomerie form with S°20.w of 2.7S. The molecular weight of this protein was estimated to be 38,000 by a short column method. The molecular shape was ...
In vitro susceptibility profile of 200 recent clinical isolates of Candida spp. to topical antifungal treatments of vulvovaginal candidiasis, the imidazole... Topical antifungal treatment of vulvovaginal candidiasis is widely recommended. The most commonly recommended topical antifungals (the imidazoles clo...
The column was washed with 30 mM imidazole, and SUMO-ETM (18 kDa band) was eluted at >90% purity with 250 mM imidazole. High molecular-weight SUMO-ETM oligomers are visible as a minor species. ETM was cleaved from the SUMO tag using SUMO protease. c, Preparative reverse-phase ...
We focus primarily on ZROP mediated by N-heterocyclic carbene nucleophiles but also discuss zwitterionic polymerizations with amidine, pyridine, and imidazole nucleophiles. The ZROP of N-carboxyanhydrides with N-hetereocyclic carbenes generates a family of functionalized cyclic polypeptoids. We can ...
New catalysts for the synthesis of highly reactive polyisobutylene: chloroaluminate imidazole-based ionic liquids in the presence of diisopropyl ether The use of [emim]Cl–AlCl3 in combination with diisopropyl ether allowed us to synthesize highly reactive polyisobutylene with an exceptionally high exo-...
simultaneously releases one of its protons to H272; therefore, H272 acts as a general base to accept the proton and to facilitate the reaction and D238 will further serve as a proton reservoir to lower the proton transfer free energy barrier and maintain a neutral imidazole group of H272....
(Cytiva). The column was washed with 20 column volumes of 25 mM Na-HEPES pH 7.5, 150 mM NaCl, 20 mM imidazole (Sigma Aldrich), and bound protein was eluted with 10 column volumes of 25 mM Na-HEPES pH 7.5, 150 mM NaCl, 500 mM imidazole. The eluate was concentrated ...