Conformational states of HRPA1 induced by thermal unfold- ing: effect of low molecular weight solutes. Biopolymers 2004;75:173-86.Carvalho ASL, Santos AM, Neves-Petersen MT, Petersen SB, Aires-Barros MR, Melo EP (2004) Conformational states of HRPA1 induced by thermal unfolding: Effect of ...
Cruz Marker™ MW Tag detection antibodies include: Cruz Marker™ MW Tag-Alexa Fluor® 488, Cruz Marker™ MW Tag-Alexa Fluor® 647, Cruz Marker™ MW Tag-Alexa Fluor® 680, Cruz Marker™ MW Tag-Alexa Fluor® 790, Cruz Marker™ MW Tag-HRP and unconjugated Cruz Marker™ MW...
Probe for a protein which is known to be of high abundance in your sample e.g. a house keeping protein Buffers may be incompatible with antibody or method of detection Check what buffers the manufacturer recommends e.g. milk blocking or BSA...
Subsequently, membranes were washed 3 times in TBS-T, then incubated with HRP-coupled secondary antibody (goat-anti-rabbit, Sigma Aldrich) in blocking buffer for 1 h at RT, and then were washed 3 times in TBS-T. Proteins were detected by chemiluminescence after incubating the membranes in...
Molecular Neurodegeneration BioMed Central Research article Open Access The PI3K-Akt-mTOR pathway regulates Aβ oligomer induced neuronal cell cycle events Kiran Bhaskar1, Megan Miller2, Alexandra Chludzinski3, Karl Herrup4, Michael Zagorski2 and Bruce T Lamb*1,3,5,6 Address: 1Department of ...
Secondary goat anti-mouse/rabbit IgG (Thermo Scientific, 1/10,000 dilution) were coupled to HRP. Membranes were developed using the PierceTM ECL kit (Thermo Fisher) with a ChemiDoc MP Imaging system (Bio-Rad, Hercules, CA). Quantification of the protein expression was performed with ImageJ ...
The multilayer films of low molecular weight dye methylene blue (MB) and horseradish peroxidase (HRP) were fabricated by layer-by-layer assembly through electrostatic force. MB was pre-absorbed with anionic polyelectrolyte poly(sodium--styrenesulfonate) (PSS). The MB/HRP multilayer film-modified Au...
Sections were subsequently hybridized overnight at 59°C with 250 ng/ml of riboprobe in hybridization solution. High stringency wash was performed, and the sections were next incubated (45 minutes) with horseradish peroxidase (HRP)-conjugated rabbit anti-DIG (1:50; DAKO, Glostrup, Denmark) in ...
(1:60,000). After washing for 3 × 15 min in TBS-Tween 20, followed by a single wash in TBS, the membrane was incubated with secondary antibodies, either anti-mouse IgG–HRP or anti-rabbit IgG–HRP (Jackson ImmunoResearch, West Grove, PA), diluted 1:20,000 in 1% non-fat milk...
(C51); sc-8020, Santa Cruz Biotechnology) at a 1:500 dilution in 2% milk/TBST solution) for 3 hours, washed 3 times with TBST, incubated with the horseradish-peroxidase-conjugated secondary antibody (goat anti-mouse IgG H and L (HRP); 1:1,000 dilution in 2% milk/TBST), and ...