V. Kuznetsova "Ferricyanide Reduction by Escherichia coli cells: Probable Contribution of Low Molecular Weight Thiols" Bioelectrochem. Bioenerg. 32 (3), p.267-75 (1993).O N Oktyabrsky, G V Smirnova, E V Kuznetsova 1993 Ferricyanide reduction by Escherichia coli cells: probable contribution of ...
Gel slices were destained using 30 mM potassium ferricyanide/100 mM sodium thiosulfate (1:1 v/v) and washed with Milli-Q NIMrwAeaHdaAtdue4/iHrc1s.eo0CTd0nhO ,MweU3ismtaSphnAoNdDt)sHiTAnw4TCHe4Nr0(Ce1 μ,O0iLrn 3emc)2su5ipMnb ematcDhtMteieTvddNeTilany/Hr.k...
The assay was performed according to the method of Oyaizu [11] with some modification. Extracts were diluted at different concentration 2.5 mL of the 0.2 mol/L phosphate buffer (pH: 7.0) and 2.5 mL of 1% potassium ferricyanide solution were added with tested sample and vortexed. The mixtures...
Following three washes in PBS (5 min each), embryos were incubated in X-Gal staining solution (10 mM TRIS–HCL, pH 7.3, 0.005% Na-deoxycholate, 0.01% IGEPAL, 5 mM potassium ferrocyanide, 5 mM potassium ferricyanide, 2 mM MgCl2, 0.8 mg/ml X-Gal, in PBS) at room temperature until ...
The gel core was diced into small pieces, destained with 1:1 v/v 30 mM potassium ferricyanide and 100 mM sodium thiosulfate, reduced in 10 mM DTT, and alkylated in 50 mM iodoacetamide in 0.1 M ammonium bicarbonate. After reagents were removed, the gel pieces were incubated with 12.5 ng/...
Measurement of Steady State Oxygen Evolution Oxygen-evolution activity of PSII was measured using a Clark-type electrode in reaction medium (0.1 m sorbitol, 5 mm MgCl2, 5 mm NaCl, 50 mm Hepes, pH 7.6) at 20 °C under saturating light. Potassium ferricyanide (2 mm), phenyl-p-benzoquinone ...
GO was prepared by a modified Hummer’s method. Ethanol, 2,2’-Azobis(2-methylpropionitrile) (≥ 99.0%, recrystallization),N,N-dimethylformamide (DMF, ≥ 99.8%, anhydrous), copper (II) chloride (CuCl2), potassium ferricyanide (K3[Fe(CN)6]), rhodamine B (RB), coomassie brilliant ...
and again washed 3 times. The material was post-fixed for 90 min in a mixture of osmium tetroxide and potassium ferricyanide in the ratio 1:1. After dehydration in an acetone series (50, 70, 90, 100, and 100%), the material was embedded in a mixture acetone–epoxy resin in the ratio...
The cells were washed with PBS twice before staining with X-gal solution [1 mg/mL 5-bromo-4-chloro-3inolyl-β-D-galactoside (Boehringer Manneheim #745-740) in N,N-dimethylformamide, 5 mM potassium ferricyanide crystalline (#P-8131), 5 mM potassium ferricyanide trihydrate (#P-3289), 2 ...
Further, 2.5 mL of 1% potassium ferricyanide, K3[FeCN6], was added to the above solution. The solution was incubated for 20 min at 50 °C and then 2.5 mL of trichloroacetic acid was added to the incubated solution, followed by centrifugation at 650 rpm for 10 min. 5 mL of distilled ...