Of note, the line width of an NMR signal is related to the molecular weight of the protein tumbling in solution. Thus, the line broadening observed during complex formation will reflect the increase in the molecular weight of the complex. In addition, the stability of the complex, that is,...
The EGFP/mCherry fluorescence intensity ratio represents the TAG suppression efficiency under varying concentrations of pAPCF or PAPd. Mean ± s.d., from three independent biological replicates. l, Fluorescence excitation (Ex) and emission (Em) spectra of ThRS(mPAPF). The fluorescence images under...
pGST-N3 was previously constructed by switching EGFP of pEGFP-N3 vector to GST using BamHIII/NotI sites. To generate N- and C-terminally deleted constructs of p62-ZZ (83–175), PCR amplified DNAs were inserted into pGST-N3 vector (Clone Tech.) using HindIII/BamHI sites. Point mutations...
B) Schematic representation of EGFP-fusion protein constructs of full length Cx55.5 (WT, I; nt1 to nt1497), carboxy terminal domain of Cx55.5 (II; CT-629; nt629 to nt1497) and 3' half of carboxy-terminal domain (III; CT-1196; nt1196 to nt1497). C) Western blot analysis of ...
EGFP assay and luciferase assay showed that 2-aminoimidazole modified PEI1800 exhibited much better transfection than PEI1800, guanidyl modified PEI1800, imidazolyl modified PEI1800 both in HEK 293 T cells and SGC-7901 cells. Besides, MTT assay showed 2-aminoimidazole modified PEI1800 exhibited ...
NR5A1-WT-EGFP-N2) was used as a template to generate four mutants (p.Q42E, p.R84C, p.R313C and p.L359_L363del). The primers used are presented in Table S1. The plasmids were transfected into 293 T cells seeded in a confocal dish with Liposomal Transfection Reagent. After 24 h...
eGFP siRNA (siCTRL), RAC1 siRNA (siRac1), Plk1 siRNA (siPlk1), and Cy5-labeled RAC1 siRNA sequences were obtained from QBI (Nez-Ziona, Israel). Long PGA was purchased from ALAMANDATM polymers (Huntsville, AL, USA). 2.2. Synthesis of PGAamine Polymers 2.2.1. Preparation of Poly-α-...
coli. The TkAAI-eGFP infusion protein was proven to exhibit alpha-amylase inhibitory activity against porcine pancreatic amylase in a suitable reaction system. Analysis of gene expression patterns proved that the relative expression level of TkAAI in seeds is highest. The results presented here ...
Mouse: Tg(Aldh1l1-EGFP,-DTA)D8Rth/J Jackson Labs Stock #: 026033 Mouse: B6.129P-Cx3cr1tm1Litt/J Jackson Labs Stock #: 005582 Mouse: B6.Cg-Tg(Nes-cre)1Kln/J Jackson Labs Stock #: 003771 Mouse: Ai6(RCL-ZsGreen) Jackson Labs Stock #: 007906 Oligonucleotides Template_Switch_Oligo: ...
The DNA-binding mutant Runx2-M was constructed by site directed mutagenesis of two arginine residues at positions 265 and 268 (GRSGRGK) known to contact DNA in the crystal structure [40]. Lentiviral plasmid for constitutive Luciferase expression pCCL-c-MNDU3c-Luc-PGK-eGFP was kindly provided ...