moles Sodium Acetate to gramsMolecular weight calculation: 12.0107 + 1.00794*3 + 12.0107 + 15.9994 + 15.9994 + 22.98977Percent composition by elementElement: Sodium Symbol: Na Atomic Mass: 22.989770 # of Atoms: 1 Mass Percent: 28.025%Element: Hydrogen Symbol: H Atomic Mass: 1.00794 # of Atoms...
MBP-Ero1 at a concentration of 30 µg/mL in 10 mM sodium acetate, pH 5.0, was immobilized at a density of 1500RU. A reference cell was prepared by blocking with ethanolamine. To analyze the binding affinity to Ero1, Osm1 or chimeric Osm1 in PBS was injected over the two flow...
The high molecular mass fractions excluded from the column (>1 kDa) were pooled for use in depolymerization experiments [64]. Enzymatic depolymerization by the same peroxidase used in the dimer degradation assays was investigated in 10 mM Na acetate (pH 4.5) containing 0.25% Tween 20, 1.5 ...
Crystals were grown using the sitting drop vapor diffusion technique (10 mg/mL protein concentration, crystallization solution: 22% w/v PEG, 20% v/v glycerol, 0.1 mol/L sodium acetate, pH 5.5). After 2 weeks, microcrystals of D76N and wt β2m were filled into 1.3 mm NMR ...
3μg of poly(A)⁺ mRNA from various mouse tissues and cell lines was denatured with formamide, fractionated on 1% agarose gels containing 0.67% formaldehyde, and electrophoresed in buffer containing 20 mM MOPS (pH 7.0), 5 mM sodium acetate, and 1 mM EDTA and then transferred to Hybond...
MS mass spectrometry PCP peridinin-chlorophyll protein PS, photosystem Q plastoquinon RC reaction centre RL red light RT room temperature SDS-PAGE, sodium dodecyl-sulphate-polyacrylamide gel electrophoresis SG sucrose gradient VCP violaxanthin-chlorophyll a binding protein Keywords Chromatic adaptation Diatom...
A desirable solvent or a coat forming agent contains methyl isobutyl ketone, methyl-ethyl keton, n-propyl acetate, isobutyl acetate, or N-methyl-2-pyrrolidone. In order to prevent the distortion of ink dots, the primer has to have a glass transition temperature (Tg) of at the lowest 60 ...
The pH was adjusted to the desired pH using glycine-HCl, sodium acetate, MES-NaOH, Tris-HCl and sodium borate buffers. The sample was equilibrated at 25°C and activity was measured using the Kunitz method. Full size image DSN has a very broad pH optimum (Figure 4B) with maximum located...
The crystals for the AaNGT-UDP-2F-Glc complex were obtained in a 0.1 M calcium acetate, 0.1 M sodium acetate pH 4.5, 10% (w/v) PEG 4000 solution. We further soaked these crystals with 30 mM UDP-2F-Glc for 5 min before flash freezing in the cryoprotectant solution. ...
The enzymatic extract (100 μL) was added to a reaction buffer containing 20 mM sodium acetate (pH 5) (J.T. Baker, Deventer, Holland, The Netherlands), 20 mM guaiacole, and 30 mM H2O2. POD was calculated by using the molar extinction coefficient of 26.6 mM−1 cm−1. The APX ...