which cleaves the C-terminal signal peptide (CSP) of precursor protein and covalently links the EtNP moiety of the mature GPI (typically in the form of the GPI core with three EtNPs and three acyl chains, Fig.1a) to the newly exposed carboxyl terminus of the so-called ω-residue (Fig...
Fluorescence analysis is a fast and sensitive method, and has great potential application in trace detection of environmental toxins. However, many important environmental toxins are non-fluorescent substances, and it is still a challenge to construct a fluorescence detection method for non-fluorescent s...
Molecular biologists have one further trick up their sleeves. Because of the repetitive, digitized nature of nucleotide sequences, computers are the ideal means of storing and processing this mass of information. Bioinformatics isa broad term first used in 1979 to describe the application of statistic...
This procedure typically resulted in a yield of ∼10 mg of Ran per 2-liter pellet. Thrombin cleavage of GST-Ran resulted in 15 non-native N-terminal amino acid residues (NH2-GSPGISGGGGGILDS). Precise cleavage by thrombin was confirmed by matrix-assisted laser desorption ionization mass ...
A set of polypeptide conjugates is expected to be able to capture a large number of phosphorylated proteins, perhaps all, and in combination with electrophoresis or mass spectrometry become a powerful tool for the monitoring of phosphorylation levels. The presented binder can easily be attached to ...
Raman spectra of aqueous Ga(III) perchlorate and nitrate solutions were measured at room temperature. From Ga(ClO 4 ) 3 solutions, a strongly polarized ba... WW Rudolph,CC Pye,G Irmer - 《Journal of Raman Spectroscopy》 被引量: 46发表: 2002年 A mass spectrometric and ab initio molecular...
Continuous synthesis of carbon-coated lithium-iron-phosphate (i) a combustible organic solution containing a mixture of lithium or a lithium compound; iron or an iron compound; and phosphorus or a phosphorous compound in an organic solvent, is fed through at least one nozzle where said ... Met...
9. The curable resin composition according to claim 8, which further comprises a condensation catalyst in the quantity within a range from 0.05 to 10 parts by mass per 100 parts by mass of said high molecular weight organopolysiloxane. 10. A cured product obtained by curing the composition ...
Finally, octamers were analyzed for mass and purity by SEC and MALDI-TOF/TOF (Supplementary Fig. 7). Octamer-containing fractions were pooled, concentrated, and diluted 50% with glycerol before long-term storage at −20 °C. Nucleosome assembly Assembly reactions were performed at 2–5...
The spliceosome executes pre-mRNA splicing through four sequential stages: assembly, activation, catalysis, and disassembly. Activation of the spliceosome, namely remodeling of the pre-catalytic spliceosome (B complex) into the activated spliceosome (Bac