Major findings of the study were: (1) the molecular mass of deduced sugarcane sucrose transporter proteins was between 53.44 and 61.80kDa, and the pIs were between 5.94 and 10.68; (2) all sugarcane sucrose transporter proteins had 12 typical transmembrane domains; (3) there were two DNA ...
Several molecular forms of human erythrocyte membrane acetylcholinesterase have been studied after crosslinking with bifunctional diimidates. The crosslinked products were analysed by centrifugation on linear sucrose density gradients containing Triton X-100. Molecular weights of covalently linked oligomers were...
Briefly, extracted hemispheres were post-fixed using 4% paraformaldehyde for 48 h, then, brains were transferred into 30% sucrose. Leica microtome (Leica; #SM210R) was used to cut 30 µm thick coronal brain sections (from the beginning of the olfactory bulb till the end of hippocampal ...
The molecular weight of the active solubilized cell-surface receptor for immunoglobulin E (IgE) was measured in nonionic detergent. The diffusion coefficient was estimated by gel filtration, the partial specific volume was estimated from the differential sedimentation in sucrose gradients prepared from H2O...
The sodB gene encoded a protein of 194 amino acids with a molecular mass of approximately 22.3 kDa that had high similarity to other prokaryotic Fe-SODs. Two enzymes with activities consistent with both these ORFs were identified by inhibition of O[sub 2, sup -]-catalyzed tetrazolium salt ...
Lamin A is a nuclear intermediate filament protein critical for nuclear architecture and mechanics and mutated in a wide range of human diseases. Yet little is known about the molecular architecture of lamins and mechanisms of their assembly. Here we use SILAC cross-linking mass spectrometry to det...
mass spectrometer (GCMS, Supplementary Figs.10and11), single crystal analysis (Supplementary Fig.12and Supplementary Data1and2), and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS, Supplementary Fig.13). Figure2a–cshows the molecular structures of PM6, ...
After centrifugation of a crude cytosol fraction from log-phase cells in a 5-20% linear sucrose gradient, polypeptides of Mr approximately equal to 185,000 and 220,000 were immunoprecipitated only from gradient fractions containing DNA polymerase alpha activity. When similar cultures were incubated ...
To measure the width of the A-site crevice74, first, the centres of mass of these nucleotide parts were calculated for each frame in each of the superimposed trajectories (see section Conformation of 23S nucleotides 2504–2506). Next, the mean position of the carbonyl atom of the ErmBL ...
Fig. 3: Distribution analysis of peripheral subunits in intact OGDHc. a Relative position of a E1o group to one vertex. The points represent individual projections of the mass of E1o along the Z-axis. The positive direction of the z-axis is defined as the direction from the origin of th...