oligonucleotide ligation and invasive cleavage based on the molecular mechanisms (Sobrino et al., 2005), and different detection methods to analyze the products of each type of allelic discrimination reaction, such as gel electrophoresis, mass spectrophotometry, chromatography, fluorescence polarization, arr...
In addition, we show that truncated products of MASTL may be catalytically active, using a cryptic C-lobe contained in a section of the NCMR, and display a different protein target palette in a HEK293 cell extract. Finally, hydrogen/deuterium exchange mass spectrometry (HDX-MS) reveal MASTL ...
P. A continuous molecular roadmap to iPSC reprogramming through progression analysis of single-cell mass cytometry. Cell Stem Cell 16, 323–337 (2015). Article CAS PubMed PubMed Central Google Scholar Hansson, J. et al. Highly coordinated proteome dynamics during reprogramming of somatic cells ...
The first identification of lysine malonylation substrates and its regulatory enzyme. Mol. Cell Proteom. 10, M111 012658 (2011). Article Google Scholar Feldman, J. L., Baeza, J. & Denu, J. M. Activation of the protein deacetylase SIRT6 by long-chain fatty acids and widespread de...
Biochemical analytical methods such as Size-exclusion chromatography (SEC), Ion Exchange Chromatography (IEX), Hydrophilic interaction chromatography (HIC), Mass spectroscopy as well as biophysical tools such as Differential scanning calorimetry (DSC), circular dichroism (CD), Fourier-transform infrared ...
We also discuss the importance of lysine residues and speculate on the possible penetration mechanism of the peptide and propose a graded-like penetration process. 展开 关键词: Degree of imidization Polyimide Evolved gas analysis-mass spectrometry ...
of 499 E3 ligase compounds [41]. From this screening, they discovered that the mRNA level of DDB1, a CUL4 adapter protein, correlated with the cytotoxicity of the CDK inhibitor (R)-CR8 (Fig.3). Subsequent quantitative proteome-wide mass spectrometry to evaluate protein abundance after ...
Lysyl hydroxylases catalyze hydroxylation of collagen lysines, and sustain essential roles in extracellular matrix (ECM) maturation and remodeling. Malfunctions in these enzymes cause severe connective tissue disorders. Human lysyl hydroxylase 3 (LH3/PLO
Amaranth seeds proteins are rich in lysine, threonine, and methionine and encrypted peptides with various biological functions which have health benefits for the consumers7. Lipids contain a high level of unsaturated fatty acids such as palmitic, oleic, linoleic, and linolenic acids and tocotrienols ...
They represent approximately 10% of all glioblastomas [2,20]. Secondary glioblastomas are described by the arginine–histidine substitution at codon 132 of IDH 1 (c.395 G > A) [2,20]. However, others express IDH 2 mutations located at codon 172, and arginine is substituted by lysine (c...