The relative molecular mass of a purified extracellular medium-chain-length poly(3-hydroxyalkanoate) (MCL-PHA) depolymerase (PhaZ(PalM4-7)) from P. alcaligenes M4-7 was 28.0 kDa, as determined by SDS-PAGE. The PhaZ(PalM4-7) was most active in 50 mM glycine-NaOH buffer (pH 9.0) at ...
In addition, an increase in subcooling, gas volume fraction, pressure, and stirring rate leads to more hydrate formation in the reactor with a constant temperature of 274.3 K. The maximum mass fraction of hydrate is observed for the case when the gas volume fraction and stirring rates are ...
1a, b). Glycosyl linkage analysis of the O polysaccharide in the HMW fraction revealed 3-substituted Rha (47.0%), 2-substituted Rha (23.6%), 2-substituted Man (18.4%), and terminal Rha (5.9%), with all sugars in the pyranose (p) configuration. In addition, gas chromatography-mass ...
Especially, some macromolecular substrates were difficult to come into contact with enzyme, which decreased the apparent activity of enzyme seriously. If enzyme molecules could be encapsulated on the surface of particle carriers, it would not only solve the problem of substrate mass transfer and ...
(Bruker, Billerica, MA, USA) for MALDI-Tof MS using α-cyano-4-hydroxycinnamic acid as the matrix. LC-MS analysis was performed using a Thermo-Finnigan Surveyor LC-MS system equipped with a PDA spectroscopic detector and an LCQ Fleet Ion Trap Mass spectrometer. Chromatographic separation was ...
The lamprey, a primitive jawless vertebrate whose ancestors diverged from all other vertebrates over 500 million years ago, offers a unique window into the ancient formation of the retina. Using single-cell RNA-sequencing, we characterize retinal cell types in the lamprey and compare them to those...
Glycosyl compositional analysis, assisted by ssNMR, demonstrated a major component of glucan (71%), chitin (9%), mannan (6%), and galactan (13%), as well as traces of chitosan and arabinan inA. fumigatus(Supplementary Table1). A gas chromatography–mass spectrometry (GC-MS) glycosyl linkage...
Mass spectrum, 150 (54), 104 m(/9z5()I,rel9, 1%)(:12)4. 01(H1) [M]+, NMR 196 (100), spectrum (DMSO-d6), d, ppm: 8.89 (3H, Harom), 3.77 (3H, N14C8H.33)(.C13,3CC5)N, M14R1.7sp(eCc1t)r,u1m27(.9DM(CS2O, C-d66),),11d8C.,6 ppm: (C4). REFERENCES 1. ...
After removing the inorganic salts and expelling the solvent, a brown mass, soluble o in acids, remains. 14 parts of this methylated compound are heated to 60'-650 C. with 8 parts of dimethyl sulfate until water solubility occurs. The quaternary compound is excellently suitable Sfor ...
where I represents the integral value of the respective signal. The molecular weight of a D unit is MD=74.1 g·mol−1and the molecular weight of a D-OH unit is MD-OH=83.1 g·mol−1, it being assumed in the latter case in purely mathematical terms that half the atomic mass of an...