(DNAJ) with CYP450 and some essential unigenes involved in the cellular respiration [glyceraldehyde 3 phosphate dehydrogenase, (GAPCP-2), cyt c oxidase (COX), cyt b reductase (cob), and NADPH dehydrogenase quinone reductase like protein], indicates shifting of other metabolic activities to ...
28,29,30]. A clinical study revealed that intrathecal administration of antisense oligonucleotides (ASO) can decrease the levels of mutant huntingtin protein (mHTT) in the cerebrospinal fluid of Huntington's disease (HD) patients, and other studies also have...
Cancer is conventionally regarded as a strictly cell-autonomous process in which gene mutations potentiate cell fitness and drive clonal competition and expansions in a selfish, competitive manner [1]. However, a growing body of evidence indicates that different tumor subpopulations may also cooperate a...
The expression levels of the tested genes were normalized to the level of gapdh (glyceraldehyde-3-phosphate dehydrogenase). Two-sided Student’s t test was used to evaluate the significance of differential expression between samples. Primers are listed in Supplementary Table 12. Three biological ...
Gene expression stability of candidate reference genes in different pig tissues analyzed by the geNorm pro- gram. Threshold for eliminating a gene as unstable was M ≥ 1.5. The respective genes and gene names are: beta-actin (ACTB), beta-2-microglobulin (B2M), glyceraldehyde-3-phos- phate ...
(Takara, Kyoto, Japan). cDNAs were employed as templates for PCR, with the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) serving as the internal reference. PCR primers for GAPDH and HTT were designed for amplification using TB Green Premix Ex Taq II (Takara, Kyoto, Japan). The CFX ...
Glyceraldehyde-3-phosphate dehydrogenase served as an endogenous reference gene. The relative quantification of gene expression was calculated using the comparative Ct (2–ΔΔCt) method. The gene-specific primer sequences utilized in the study are as follows: PTGS2: forward, 5′-CCTTCACCTCGATGC...
The EC(s) used in these studies were let-7a and miR-16 [24], U6 small nuclear RNA (snRNA) and tRNA for initiator methionine [25], 18S rRNA [26], glyceraldehyde-3-phos- phate dehydrogenase (GAPDH) [27] and in one article, the EC used for RQ-PCR analysis was not given [28]. ...
This is caused by some impurities during product oxidation of glycerol for example glyceraldehydes, dihydroxyacetone and also free fatty acids (Yong et al., 2001). The alcohol group (C-OH) also appears in treated and commercial glycerol at a spectra value of 1015.2800 cm-1 and 1038.5400 351 ...
We constructed pMAPR-1 which carries cDNA coding for the Pr1 protease cloned behind the constitutive Aspergillus nidulans glyceraldehyde-3-phosphate dehydrogenase (gpd) promoter. pMAPR-1 was constructed using standard procedures (Sambrook, J., Fritsch, E. F. and Maniatis, T. in Molecular Cloning ...