(数据和曲线)快速查看,如下图所示: Molecular Devices -6- MolecularDevices,400-820-3586 数据比较界面 (Comparison),点击此按键后可以将各个数据组信息拖拽至右侧的比较工作 区域中,方便数据查看和比较,如下图所示: 多任务流程界面 (Workflow),点击此按键后可在右侧流程界面下监视所有工作流程状态, 方便设置多小时...
The standardized bacterial suspension (0.1 ml) was micropipetted into a 96-well microtiter plate and maintained at 37 °C for 8 h before 0.1 ml of the test compounds and controls, in triplicates in the various investigated concentration ranges (MIC to 1/8 MIC) were transferred into each wel...
You should see DNA of much higher molecular weight in comparison to the marker. Alternatively, a plasmid that has been cut with a single restriction enzyme may also be used as a ligation control. This sample may then be used in transformation as a transformation control. LigaFast Rapid DNA...
After incubation at 30 °C for 48–72 h, the cultures were resuspended and the OD600 recorded using Spectramax plate reader (Molecular Devices). NHA2 purification S. cerevisiae strain FGY217 was transformed with the respective vector and cultivated in 12l cultures containing -URA medium and ...
Absorption was measured at λ = 595 nm in a SpectraMax ABS plate reader (Molecular Devices). The data for every cell line were first blanked to those of control wells coated with only BSA and then corrected for the plated cell count obtained from poly-l-lysine-coated wells. Finally...
The comparison of molecular weight distribution of protein hydrolysates and NJSP from the skin of Nibea japonica was shown in Fig. 2(b). The protein hydrolysates from the skin of Nibea japonica mainly consisted of peptides with lower MW (72.33% of the fraction below 3 kDa), and the other...
underway is to annotate the sequence with all its elements including genes, regulatory elements, repeats and duplications. Additional tasks include the comparison between the human sequence and that of other organisms. As mentioned above, one of the most surprising findings is that the estimated ...
Calcium-mediated changes in fluorescence were monitored in a Fluorometric Imaging Plate Reader (FLIPR) Tetra instrument (Molecular Devices, USA). Fluorescence intensity was measured for 12 s to establish baseline fluorescence before addition of the test compound (20 µL/well) and further readings ...
13Cα and 13Cβ chemical shifts were calculated in presence of ΔNt-Tau or pLK to measure possible changes in secondary structural trends upon comparison to the chemical shifts of αS in a pure statistical coil conformation68 (Supplementary Fig. 5c). R1ρ rates were measured by recording ...
Absorbance was subsequently read at 492 nm with reference wavelength at 690 nm, using a VersaMax plate reader (Molecular Devices). ERK activation analysis. NIH 3T3 cells were transfected with pcDNA3 plasmid (expressing GM3764 or GFP) and after 48 hours were lysed in RIPA buffer ...