Cloning and/or sequencing Yes No No Yes Yes PCR-based Usually no Yes Yes Yes Yes Radioactive detection Usually yes No Yes or no Usually no No Reproducibility/reliability High Low High High High Effective multiplex ratio Low Moderate High High Moderate to high Marker index Low Moderate Mode...
(2) the biologic characteristics of the mouse obesity mutations; (3) our progress in the use of positional cloning techniques to clone the mouseobese (ob)anddiabetes (db)genes; (4) an approach to polygenic obesity in mice; and (5) the possible relevance of the mouse obesity mutations to ...
restoration of potential species which may be vulnerable in the near future and conservation of closely related species for the possibility of exploring useful bioactive molecules present in them. These molecular approaches include both non-PCR- and PCR-based techniques. PCR-based techniques have been ...
PCR, cloning and sequencing was performed as mentioned earlier and each consensus sequence was analyzed50,51. The nucleotide and deduced amino acid sequences were characterized using the same bioinformatics tools as mentioned earlier. Response of TIFY TF to biotic and abiotic stresses...
Cloning, plasmid construction, and mutagenesis LRRK2RCKW and Rab8a protein expression vectors were cloned as previously described35. The LRRK1 sequence was codon optimized for expression in Spodoptera frugiperda (Sf9) cells and synthesized by Epoch Life Science. The DNA coding for WT LRRK1 residue...
Cloning strategy for reporter constructs. The constructs containing the Caenorhabditis elegans Ce-pdk-1 and the Haemonchus contortus Hc-pdk-1 promoters (pL-Cepdk and pL-Hcpdk) were made in the vector pPV199 [34]. Briefly, the Ce-pdk-1 promoter region was cloned into pPV199 (BamH1 and Age...
The amplified DNA fragments were purified from agarose gels using UltraClean 15 DNA Purification Kit (MO Bio, CA, USA), and subsequently cloned into the TOPO TA Cloning Kit (Invitrogen, CA, USA). Two clones of the eukaryotic SSU rRNA gene, from each of the two isolates (i.e., four ...
Hsu T, Hutto DL, Minion FC, Zuerner RL, Wannemuehler MJ: Cloning of a beta-hemolysin gene of Brachyspira (Serpulina) hyodysenteriae and its expression in Escherichia coli. Infect Immun 2001, 69: 706–711. 10.1128/IAI.69.2.706-711.2001 Article PubMed Central CAS PubMed Google Scholar Rahma...
Tsai-Pflugfelder M et al (1988) Cloning and sequencing of cDNA encoding human DNA topoisomerase II and localization of the gene to chromosome region 17q21-22. Proc Natl Acad Sci USA 85:7177–7181 CASPubMedGoogle Scholar Capranico G, Tinelli S, Austin CA, Fisher ML, Zunino F (1992) Diff...
2012). Several QTLs were identified for GY-related traits on maize chromosomes, which will also provide targets for gene cloning and QTL pyramiding (Tuberosa and Salvi 2009). On the other hand, using QTL analysis in maize enabled Li et al. (2015) to reveal the genetic correlation between ...