targeted bio-psy, polymerase chain reaction (PCR) and microbio-logical evaluation, oncogynecological ultrasound and CT examination were done. Clinically, the lesion acted as an advanced cyst. However, no malignant cells had been recognized into the bio-psy while the histopathological finding corresponde...
Conclusion The heterogeneous nature of HNSCCs has hindered the identification of specific targets for the development of targeted therapies. Over the past 30 years we have developed a better understanding of the genomic, proteomic, microbiomic and metabolomic alterations in HNSCCs. This knowledge is h...
Next, using ultraviolet (UV) gel documentation equipment (UVP Bio-Doc IT-220 Imaging System, BioExpress, USA), the DNA bands were immediately observed and photographed. The PCR products were purified using the DNA Clean and Concentrator Kit (Zymo Research, USA) in accordance with the ...
Bacterial identification was initially performed by matrix assisted laser ionization time of flight mass spectrometry (MALDI-TOF MS) using the Vitek®MS (bioMérieux, Marcyl'Étoile, France) and the identifica- tion of A. baumannii at species level was confirmed by real time PCR of the ...
® soil DNA Kit (Omega Bio-tek, Norcross, GA, USA). The hypervariable region of the bacterial 16S rRNA gene (V3-V4) was amplified with primer pairs 338F (5′-ACTCCTACGGGAGGCAGCAG-3′) and 806R (5′-GGACTACHVGGGTWTCTAAT-3′) by an ABI GeneAmp® 9700 PCR thermocycler (ABI, ...
Biochem Bioph Res Commun, 2014, 465: 433–436. Google Scholar [39] Gao Q, Xiao Y, Zhang C, et al. Molecular characterization and expression analysis of toll-like receptor 2 in response to bacteria in silvery pomfret intestinal epithelial cells. Fish Shellfish Immunol, 2016, 58: 1–9...
e.g.Melanoides tuberculataandTerebralia palustris[36]. Renewed interests in Hc structural complexities and the assemblages of associated sugars and lipids aim to exploit the vast therapeutic potential of these megamolecules (Table1). Especially from molluscs, Hcs are tested for application as bio-adju...
Sudarshan M, Sundar S. Parasite load estimation by qPCR differentiates between asymptomatic and symptomatic infection in Indian visceral leishmaniasis. Diagn Microbiol Infect Dis. 2014;80(1):40–2.https://doi.org/10.1016/j.diagmicrobio.2014.01.031. ...
The Mitis group of streptococci includes an important human pathogen, Streptococcus pneumoniae (pneumococcus) and about 20 other related species with much
Diagnosis of leishmaniasis has always been a major challenge as its clinical features resemble some other commonly occurring diseases such as tuberculosis, typhoid, and malaria. Reliable laboratory methods become important for differential diagnosis. Demonstration of the parasites in stained preparations of...