Eyelids were fixed in Zamboni’s fixative containing 1.6% paraformaldehyde, <1.5% picric acid, mono- and disodium buffer salts, pH 7.3, 0.1 M (Australian Biostain Pty Ltd., Victoria, Australia) overnight at 4 °C, and then washed in PBS. Eyelids from the right eye were incubated over...