The catalytic activity test in the esterification reaction of palmitic acid with methanol was conducted by bath at 65 掳C with a variation of reaction time of 1, 2, 3, 4 and 5 h. Catalytic activity has been observed qualitatively using GC-MS and quantitatively by changes in acid number. ...
A variety of PTMs have greatly expanded the functional field of proteins, which is particularly important in the immune recognition of tumor therapy. In particular, many peptides presented to T cells through histocompatibility complex are post-translational modified. The regulation of this process may...
After the same time, 100 ± 13% of alkyne palmitic acid 16:0;Y was still present, and 88% thereof remained in its original form. Modifications produced mostly desaturated FA 16:1;Y and 18:1;Y. The PUFA 18:2;Y did not show significant loss of material. About 20–30% of the ...
图 1.4 棕榈酸对 HepG2 细胞内脂质积聚的影响 A:油红 O 染色;B:细胞内 FFA 的定量测定(mean±SD,n=4,*:P0.05 vs Control);C:荧光 显微镜动态观察 FFA 摄取 Figure1.4 Effect of palmitic acid on lipid accumulation in HepG2 cells. A: Oil Red O staining;B:FFA content tested by ELISA kit(...
For analysis of protein-bound fatty acids, 20 μl of internal standard solution (4 nmol of [2H11]hexanoic acid and 10 nmol of [2H4] palmitic acid in methanol) was added to the remaining sample (∼4 mg of protein), which was then diluted with an equal volume of concentrated HCl and...
In addition, the two forms of the protein were found to be modified by a glycophospholipid, components of which were characterized by metabolic incorporation into placental alkaline phosphatase of 3H-labeled compounds such as myo-inositol, palmitic acid, stearic acid, mannose, glucosamine, and ...
Hashizumeet al.modified the side chain of insulin molecule with palmitic acid, and the lipophilicity of palmitoyl insulin was improved. The researchers labeled insulin with isotopes and inferred the content of insulin in plasma by measuring the radioactivity in plasma within 6 hours after administration...
The main goal of this research effort was to engineer butterfat so as to increase its level of unsaturated fatty acid residues (viz. oleic acid) and concomitantly decrease its level of medium-to-long chain saturated fatty acid residues (viz. myristic and palmitic acids). All reactions were ...
Free fatty acids inhibit insulin signaling by inhibiting AKT kinase activity8; unsaturated C18 fatty acid elaidate suppresses the insulin-induced fusion of GLUT4 storage vesicles in the plasma membrane9, and palmitic acid induces sustained JNK activation and insulin resistance in primary mouse ...
such as stearic or palmitic acid, but unsaturated acids such as oleic or branched carboxylic acids such as napthenic acids of molecular weights of from about 200 to 500, or synthetic carboxylic acids, are preferred, because of the improved handling and solubility properties of the resulting coppe...