The multi-site attachment of polymers and the high values of solute acceleration factor S (see Eq. (4.21) have the consequence that small differences in eluent composition can cause dramatic changes in elution behaviour, see Sect. 4.3. For example, a polystyrene standard (M = 50,000) was ...
Related to Mobile phase:Stationary phase,HPLC el·u·ent alsoel·u·ant(ĕl′yo͞o-ənt) n. A substance used as a solvent in separating materials in elution. [Latinēluēns, ēluent-, present participle ofēluere,to wash out; seeelute.] ...
HPLCRPLCmetal-dipivaloylmethane additive systemsIt is likely that 90% of all small-molecule high-performance liquid chromatography (HPLC) separations can be done with a hydrocarbon-bonded phase of C4, C8, or C18 length using various mobile phases. The remaining 10% of the cases are more ...
through the distribution of the analytes between a stationary phase packed in a column and a liquid mobile phase that is pumped through the column. Traditionally, the stationary phase was polar, e.g., silica, and the mobile phases used were relatively non-polar. This HPLC mode is called[...
Phase ratio Φ for an HPLC column is a common parameter being defined as the ratio between the volume of the stationary phase Vst and the void volume of the column V0. Although apparently simple, the evaluation of phase ratio presents difficulties because there is no sharp boundary between the...
Vinay Kumar Pandey, in Introduction to Biomedical Instrumentation and Its Applications, 2021 Mobile phase The liquid phase is pumped to the stationary phase column at a constant speed. Before entering the column, the sample is introduced into the carrier flow. When they reach the column, the ...
mobile phase是什么意思 释义 (色谱分析的)流动相; mobile phase 例句 1.In adsorption chromatography, solute molecules of the mobile phase undergo many successive adsorptions and desorptions. 在吸附色谱中,流动相的溶质分子进行多次连续的吸附和脱附。
stationaryphaseandCmisitsconcentration inthemobilephase. Principle ThelevelofInteraction(adsorption) packingmaterial&sampleA,Bdiffers, resultingindifferentspeedsoftravelofA& Binamedia(paper,columnetc.) Usuallysampletobeanalysedisinjected intoacarrier(gasorliquid) ...
Supercritical fluid chromatography using n-pentane as a mobile phase and Porasil C as a column packing was applied to the characterization of Dow-Corning 710 fluid, a commonly used gas chromatographic stationary phase. Under the experime... JA Nieman - 《Separation Science》 被引量: 88发表: 19...
B.,Vonach,and,G.,Schomburg 摘要: Summary By reversed-phase high-performance liquid chromatography (HPLC) on chemically bonded phases using common mobile phases (methanol-water, methanol-isopropanol, water-acetonitrile) containing Ag+, separations with considerably increased selectivity for various types ...