For polysomal fraction RT–qPCR analysis, RNA was extracted from each sucrose fraction by standard Trizol method. Five nanograms of polyA þ synthetic luciferase mRNA (Promega) was spiked into the RNA of each fraction for normalization (following methods established by Thoreen et al.38). All ...
显然,THINKPAD的工程师们考虑到了THINKPAD用户有可能需要在已安装了WINDOWS XP的情况下切换到AHCI模式,所以专门提供了一个脚本用来避免在STANDARD IDE切换到AHCI模式后出现蓝屏,这个脚本放在C:\DRIVERS\WIN\IMSM\PREPARE目录下。由于这个脚本是专门提供给THINKPAD笔记本使用的,所以必须先修改一下才可以用在...
HTTP and FTP Auth or non-standard port: deb http://user:pass@example.com:8080/debian stable main contrib non-free HTTPS with sending Basic HTTP authentication information (plaintext username and password) for all requests: (this was default behaviour of Wget 1.10.2 and prior and is need...
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