The protocol also includes directions for successful pairing with multiplex sequencing reactions. Conclusions We validated our method by comparing the experiment-to-experiment variation between biological replicates of chromatin preparations from S. cerevisiae. Results from our matched preparation consistently ...
核小体是染色质复杂三维结构的基本单位,它在基因组上的定位及占位在DNA转录、复制和修复等基础生物过程中发挥重要功能。在众多核小体定占位研究技术中,微球菌核酸酶测序(micrococcal nuclease sequencing, MNase-seq)被认为是目前最为高效的方法,因此应用十分广泛。研究人员利用该技术绘制了多种生物的核小体图谱,并揭示...
This protocol describes single-cell micrococcal nuclease sequencing (scMNase-seq), a method for detecting genome-wide nucleosome positioning and chromatin accessibility simultaneously from a small number of cells or single cells. To generate scMNase-seq libraries, single cells are isolated by FACS ...
PlantsThe precise positioning of nucleosomes along the underlying DNA is critical for a variety of biological processes, especially in regulating transcription. The interplay between nucleosomes and transcription factors for accessing the underlying DNA sequences is one of the key determinants that affect...
While several independent techniques can be performed and then analyzed in composite to reveal this chromatin landscape, the use of micrococcal nuclease (MNase) digestion can resolve the footprints of nearly all chromatin proteins simultaneously. The protocol below describes the use of MNase to identify...
Eukaryotic DNA is wrapped around histone octamers, known as nucleosomes, in an orderly fashion that provides the primary structure of chromatin organization. The compaction of DNA into nucleosomal repeats not only allows the tight packaging of the large eukaryotic genomes into the nucleus, it also ...
Richárd BártfaiCharacterization of the Nucleosome Landscape by Micrococcal Nuclease-Sequencing (MNase-seq). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Wieteke Anna Maria Hoeijmakers and Richa´ rd Ba´ rtfai...