Cell clus- ters were disrupted by pipetting and passing the suspension through a 40 μm cell strainer. The resulting cell sus- pension was centrifuged (10 min, 400 × g) and the cell pellet was resuspended in complete macrophage medium (CMM) containing 30% L929 cell conditioned...
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The cells were extracted with PBS with 1% Triton X100. The conditioned media and the cell extracts were dot blotted onto nitrocellulose paper. The blot was blocked with 5% non-fat dried milk in PBS. Mab M75 were used to detect the MN 20-19 protein in the dot blots. A rabbit anti-...
Abstract Bacterial wilt (BW) is a serious disease that affects eggplant (Solanum melongena) production. Although resistance to this disease has been reported, the underlying mechanism is unknown. In this study, we identified a NAC family transcription factor (SmNAC) from eggplant and characterized i...
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2.4.4. Live/Dead Analysis via Fl Cytometry For CdSe/ZnS-MPA QDs at the 2 concentrations (6.25 and 25.0 µg/mL) with the lowest viability via the MTT assay, we also assessed cell viability using the LIVE/DEAD™ Cell Vitality Assay Kit, C12 Resazurin/SYTOX™ Green (L34951, ...