Lett. 370https://doi.org/10.1093/femsle/fnad049 (2023). Hugerth, L. W. et al. DegePrime, a program for degenerate drimer design for broad-taxonomic-range PCR in microbial ecology studies. Appl. Environ. Microbiol. 80 (16), 5116–5123. https://doi.org/10.1128/AEM.01403-14 (2014)...
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Nucleic Acids Research 14,9 qRT-PCR Enhancer-promoter interactions can form independently of genomic distance and be functional across TAD boundaries. Nucleic Acids Research 14,9 qRT-PCR Biochemical and cellular insights into the Baz2B protein, a non-catalytic subunit of the chromatin remodeling compl...
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2.16. Quantitative real time-polymerase chain reaction (qRT-PCR) The total RNA in the cells was extracted using Trizol (SigmaAldrich, USA) and then quantified using nanodrop 2000 (Thermofisher, USA). RNA was then reversely transcribed into cDNA and then mixed with SYBR Green Mastermix and prim...
6f and g, RT-PCR and western blot analysis results demonstrate that the MnARK nanovaccine can indeed activate the cGAS-STING pathway in DC2.4 cells to enhance RBD-specific humoral and cellular immune responses. Such a conclusion is also consistent with the data of our transcriptome analysis and...
本研究探讨中国汉族人群MN血型相关基因gypa的多态性.随机选取中国汉族人群中无血缘关系的无偿献血者202人份,以血清学方法鉴定样本的fu血型表现型.根据GenBank的NG-007470基因参照序列设计gypa第2外显子引物,对202人份的DNA进行ICR扩增,同时对扩增产物进行直接测序.结果表明:所有样本的gypa第2外显子第1,56...
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confirmed by qRT-PCR assay that the level of MNX1-AS1 was significantly elevated in LSCC tissues compared with normal tissues, and the expression level was higher at advanced tumor stage. The expression level of MNX1-AS1 was positively correlated with T stage (P < 0.009), lymphatic ...
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