5 ARTICLE MnSOD β-Actin MnSOD 150 ***P<0.001 100 50 0 NATURE COMMUNICATIONS | DOI: 10.1038/ncomms7053 30 kDa MnSOD 20kDa 50 kDa 40 kDa β-Actin Control scrRNA siRNA MDA-MB231 20kDa 40 kDa AMPKpi (T172) **P<0.01 150 100 50 0 Glycolysis 4 ATP 100 3 ** ** 2 50 1 00 ...
The proteins in the 70-kDa Mn(II)-oxidizing band were also identified by nano LC-MS/MS, and the peptides, generated by trypsin digestion, also showed a high matching score (score: 34.16; coverage: 6.63) with the bilirubin oxidase (WP_056084337) in strain Arthrobacter sp. Leaf137 (...
图5.PLA2R抗原表位发现历程。 除了PLA2R,MN中还发现了其他抗原,如NEP和THSD7A。在患有NEP缺乏症的孕妇中发现了针对NEP的同种异体免疫抗体,导致胎儿肾小球基底膜(glomerular basement membrane,GBM)中出现免疫复合物。THSD7A是一种250 kDa的蛋白质,存在于PMN患者的血液样本中,这些患者的抗PLA2R1抗体检测呈阴性。在...
And 63-kDa isoforms iPLA2γ are distributed in the peroxisomes guided by a peroxisomal localization signal peptide near the C-terminus [47]. PLA2γs are mainly localized in mitochondria and peroxisomes which are also the two main subcellular organelles that produce H2O2. Therefore, we speculate ...
Vivaspin concentration tubes (cutoff, 5 kDa) were purchased from Vivascience (Hannover, Germany). 10-20% Criterion Tris-Tricine gels were obtained from Bio-Rad. Isopropyl-β-D-1-thiogalactopyranoside (IPTG) free of dioxane and Agarose were purchased from Saveen Werner AB. Phenylmethylsulphonyl...
5.通过微针渠道递送富血小板血浆(PRP)做法是错误的。微针滚动产生的深度有限。皮肤科常用的微针是简单的、无涂层的微针,针长为 1.5mm,而微针刺人皮肤的深度是针体长度的 1/3。在 PRP 中的所有生长因子的分子量都在kDa 以上,大于500Da(1kDa=1000Da)。 PRP 是亲水性的,不能通过富含脂质的疏水性角质层。因此...
They are of low molecular weight (6-7 kDa) and of the 60+ ... M Aschner,T Syversen,DO Souza,... - 《Experimental Biology & Medicine》 被引量: 102发表: 2006年 Effect of Aegle marmelos on biotransformation enzyme systems and protection against free-radical-mediated damage in mice. The ...
Human erythrocyte Mn(2+)-dependent (C'A') and -independent (CA) protein-serine/threonine phosphatase (PP) 2A are composed of 34-kDa catalytic C' and C subunits, in which the metal dependency resides, and 63-kDa regulatory A' and A subunits, respectively. Each catalytic and regulatory subu...
(Fig.4e), all image stacks were monitored at a typical depth of 120–164 μm from the surface. To monitor the vessel leakiness, we preinjected 70 kDa Texas red i.v. to assess vessel integrity following the cranial window procedure (red signals in Fig.1f). Only red fluorescence ...
Two components of the repressor complex (35 and 42 kDa) were found to be in direct contact with PR4 by UV cross-linking. Increased cell density, known to induce MN expression, did not affect levels of PR4 binding in HeLa cells. Significantly reduced repressor level seems to be responsible ...