将匀浆以10,000rpm离心以沉淀蛋白质和蛋白质结合的有效负载。然后将上清液进行固相萃取,通过LC-MS检测MMAE和MMAF的信号。 MCE尚未独立确认这些方法的准确性。它们仅供参考。
相同的lc-ms/ms系统亦可被使用。 [0062] 实施例2液质检测条件 [0063] 1、液相色谱条件 [0064] 色谱柱:agilent hilic plus,3.5μm,柱规格为2.1*100mm;色谱柱温:40℃;流动相a:h2o/fa/1m hcoonh4=100/0.1/1(体积比);流动相b:乙腈;洗液:乙腈/水的体积比为80/20;自动进样器温度为4℃;梯度洗脱,流速为...
LC-MS is used to measure the concentration of MMAE in a parallel cohort of L-82 tumors with an identical treatment regimen. Although tumor volume is not different among treatment groups 3 days after dose, the intratumoral MMAE measurement reveals two patterns. First, intratumoral MMAE ...
LC-MS is used to measure the concentration of MMAE in a parallel cohort of L-82 tumors with an identical treatment regimen. Although tumor volume is not different among treatment groups 3 days after dose, the intratumoral MMAE measurement reveals two patterns. First, intratumoral MMAE ...
(monomethyl auristatin E, MMAE). ADC-mediated cytotoxicity is not related to target expression or drug: antibody ratio. LC-MS was used to measure the concentration of MMAE in the L-82 tumor parallel cohort by the same treatment protocol. Although there was no difference in tumor volume ...
LC-MS is used to measure the concentration of MMAE in a parallel cohort of L-82 tumors with an identical treatment regimen. Although tumor volume is not different among treatment groups 3 days after dose, the intratumoral MMAE measurement reveals two patterns. First, intratumoral MMAE ...
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• 提供 LC/MS、NMR、HPLC、手性分析、元素分析等各项质检报告,确保产品的高纯度、高品质;• 产品的生物活性多经各国客户实验验证;• Nature, Cell, Science 等多种顶级期刊及制药专利收录了MCE客户的科研成果;• 专业团队跟踪最新的制药及生命科学研究进展,为您提供全球最新的活性化合物;• 与世界各大...
• 提供 LC/MS、NMR、HPLC、手性分析、元素分析等各项质检报告,确保产品的高纯度、高品质; • 产品的生物活性多经各国客户实验验证; • Nature, Cell, Science 等多种顶级期刊及制药专利收录了MCE客户的科研成果; • 专业团队跟踪最新的制药及生命科学研究进展,为您提供全球最新的活性化合物; ...
• 提供 LC/MS、NMR、HPLC、手性分析、元素分析等各项质检报告,确保产品的高纯度、高品质; • 产品的生物活性多经各国客户实验验证; • Nature, Cell, Science 等多种期刊及制药收录了MCE客户的科研成果; • 专业团队跟踪*新的制药及生命科学研究进展,为您提供全球*新的活性化合物; ...