To confirm the function of AvmM, we overexpressed AvmM inEscherichia coliBL21(DE3) and purified it to homogeneity with an N-terminal His6-tag (Supplementary Fig.9a). Incubation of3with AvmM in the phosphate buffer (pH 7.0) led to the conversion to2, whereas the control reaction using boi...
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Hi! Sorry to make this an issue, but I'm running into it! I've followed the README and am trying to get it running but I run into quite a few errors. Maybe I'm just missing a dependency or something like that, but I haven't quite figured...
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Carbon raisermeans a substance containing carbon added in thesmelting of steel and ironfor making up the carbon content lost. In molten iron, factors such as the smelting time, holding time, and longer overheating time lead to the carbon content decreases. ...
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