从 5' 到 3'(通常显示为“左”到“右”),基因组包含 GAG、POL和ENV区域,编码结构蛋白,酶包括 RNA 依赖性 DNA 聚合酶(逆转录酶)和外壳 蛋白质,分别。 除了这三种多聚蛋白:GAG、POL和ENV——所有逆转录病毒都很常见,MLV 还产生p50/p60蛋白,这些蛋白由其基因组 RNA 的选择性剪接产生。病毒基因组分子包含 ...
逆转录酶具有Mg2+依赖性,该酶的催化活性对Mg2+浓度非常敏感。 蛋白特点:基因名称gag-pol(来源:鼠白血病病毒);蛋白名称:M-MLV逆转录酶:671个氨基酸,相对分子质量约为76.1kDa。 逆转录过程: 逆转录酶的作用是以dNTP为底物,以RNA为模板,tRNA(主要是色氨酸tRN...
Methods The gag-pol gene of Moloney murine leukemin virus (MoMLV) were amplified by RT-PCR,they were subcloned into eukaryotic expression vector pcDNA4/HisMaxA.The recombinant plasmid pcDNA4/HisMaxA-gag-pol was thus constructed and identified by sequencing and digestion with restriction enzymes. ...
They all contain three major genes: gag, pol and env, which encode structural proteins and enzymes. Complex retroviruses, like the human immunodeficiency virus type-1 (HIV-1), encode additional regulatory and accessory proteins not found in simple retroviruses like murine leukemia virus (MLV). ...
Results Using FLY cells, which express the MLV gag and pol genes, we generated stable producer cell lines that express the HIV-1 envelope gene and a retroviral vector genome encoding the green fluorescent protein (GFP). The BH10 or 89.6 P HIV-1 Env was expressed from a bicistronic vector ...
除了这三种多聚蛋白:GAG、POL和ENV——所有逆转录病毒都很常见,MLV 还产生p50/p60蛋白,这些蛋白由其基因组 RNA 的选择性剪接产生。病毒基因组分子包含 5' 甲基化帽结构和 3' 聚腺苷尾。 基因组包括一个保守的 RNA 结构元件,称为核心衣壳化信号,指导 RNA 包装到病毒粒子中;该元件的三级结构已使用核磁共振波谱...
Improved vectors are characterized by the presence of deletion in encoding regions MLV gag, pol and env that ensures to avoid the homologous recombination. Said vectors provide safety and effective expression of foreign genes. Vectors can be used in genetic therapy. EFFECT: improved and valuable ...