MLO05 Developmental outcome in five children with M. Hurler after stem cell transplantation with fludarabine based preparative regimendoi:10.1016/S1090-3798(08)70358-1T.LückeandA.DasandH.HartmannandK.W.SDOSEuropean Journal of Paediatric Neurology
In all cases, the lowest limit of significance (e-value) for any potential hits was set at 1e−20. All potential MLO sequences were systematically validated by reciprocal BLAST on the M. truncatula JCVI Mt4.0 (http://jcvi.org/medicago/index. php) and A. thaliana TAIRv10 databases (...
Plants were spray-inoculated with coni- dial suspension (5 × 105 spores ml−1), covered in sealed propagators to maintain 100% humidity and incubated in a controlled environment (16 h photoperiod, 80 μmol m−2 s−1, 25 °C). Methods for Trypan blue-lactophenol staining...
Plants were spray-inoculated with coni- dial suspension (5 × 105 spores ml−1), covered in sealed propagators to maintain 100% humidity and incubated in a controlled environment (16 h photoperiod, 80 μmol m−2 s−1, 25 °C). Methods for Trypan blue-lactophenol staining...
Per family, five plants (positive for the presence of the overexpression construct) were randomly chosen to measure the expression of the transgene using qRT-PCR (Additional file 1). It was found that the transgene expression in both CsaMLO8 T2 families was signifi- cantly higher than in the...
fragments for the qPCR analysis. ForwardReverse Name(′5 - ′3)(′5 - ′3) EF1αGAACTGGGTGAACCAAAATATC CTTGATAGGCCGGAGTAAAAGA (SEQ ID NO: 7)(SEQ ID NO: 8) GAPDHTTCTCGTTGAGCCACAGACTTC GGCTATTCCAATCGGTGACA ...
(0% host cell entry; Fig.1Eand Table2). This minute yet reproducible difference in host cell penetration could be due to residual levels of truncatedMLOmRNA in themlo2-6mlo6-4mlo12-8 mutant. This concerns particularlyMLO6, for which the T-DNA insertion site in themlo6-4 mutant is at...
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