Here, we investigated the MLL-AF9 function by means of an antisense phosphorothioate-oligodeoxyribonucleotide (MLL-AF9-PS-ODNas) using the THP-1 AML-M5 cell line carrying t(9;11). Having confirmed that MLL-AF9-PS-ODNas induces strong inhibition of THP-1 cell growth, but only a...
However, in the murine MLL-AF9 cell line, Pol II phosphorylation was not affected by AT7519 at concentrations that completely inhibited phosphorylation of Pol II in MOLM13 cells. When the drug concentration was increased to an extremely high dose of 2000 nM, levels of not only Ser 2, but ...
Objective: Clone the MLL-AF9 fusion gene from the acute monocytic leukemia cell line THP-1 and analyze its junction sequence. Methods: RT-PCR was used to separate MLL-AF9 fusion gene from the acute monocytic leukemia cell line THP-1 and clone it into T vector for sequence analysis. ...
2003; 22: 8671-6 [9] Kawagoe H and Sano K, Target down-regulation of MLL-AF9 with antisense oligodeoxyribonucleotide reduces the expression of the Hoxa7 and A10 genes and induces apoptosis in a human leukemia cell line, THP-1. Leukemia 2001; 15: 1743-1749 [10] Thompson A, Quinn MF...
【关键词】融合基因类,MLL—AF9;基凶,p27;基因表达;细胞系,THP-1EffectofMLL-AF9fusiongenesilenceofacutemonocyticleukemiacelllineTHP-1oncyclin-depend-entkinaseinhibitorp27expressionL/Lei,UULing—bo,WANGLi,ZOUPing.InstituteofHematology.UnionHospital,TongiiMedicalCollege,HuazhongUniversityofScienceandTechnology...
In the AML cell line THP1 which harbors this t(9;11) translocation, endogenousMLL-AF9was silenced via siRNA while ensuring specificity of the knockdown and its efficiency on functional protein level. Results The differential gene expression profile was validated for leukemia-association by gene set...
Targeted down-regulation of MLL-AF9 with antisense oligodeoxyribonucleotide reduces the expression of the HOXA7and -A10 genes and induces apoptosis in a human leukemia cell line, THP-1 H Kawagoe, R Kawagoe & K Sano Leukemia volume 15, pages 1743–1749 (2001)Cite this article 1268 Accesses...
a human leukemia cell line expressingMLL-AF9, were treated with antisense oligodeoxyribonucleotide (ODN) complementary to the coding sequence of theMLL-AF9junction. Down-regulation of theMLL-AF9transcript was accompanied by the reduced expression of theHOXA7and-A10genes, but not of theHOXA2, -...
Cytoge- netic and molecular analysis of the acute monocytic leukemia cell line THP-1 with an MLL-AF9 translocation. Genes Chromosomes Cancer. 29:333-338. https://doi.org/10.1002/1098-2264(2000)9999:9999%3C:: AID-GCC1040%3E3.0.CO;2...
抑制mll-af9融合基因表达对急性单核细胞白血病细胞THP-1增殖的影响 Effect of Down-regulating mll-af9 Gene Expression on Proliferation of Acute Monocytic Leukemia Cell Line THP-1;