MLL/AFXl、MLL/IP、MLL/1Q和MLL.PTD等11种。 1.2流式细胞技术采用活细胞三色荧光标记检测的免疫表型。 1.3对部分AL患者作染色体核型分析。 2 B,C比值法的建立 2.1 B区和C区引物设计 在正常RARa、AMLI和MLL的cDNA模板上,分别针对RAR0t、和MLL基因的断 ...
In case of the detection of MLL rearrangements by Southern blot analysis the intragenic duplication was confirmed by single round RT-PCR and sequencing of the PCR-product. Based on the current results the incidence of MLL PTD is 7/99 (7%). First data of clinical outcome underline the results...
MLL—PTD;②受中期细胞数的限制,当细胞数量太少 时,不能进行核型分析;③特异性不强,很少能区分 开含有MLL 基因重排的1lq23 和不含有MLL 基因 的11q23 一q25C].FISH 分析则受到探针长度的限制, 不能在每个细胞里都检测到信号,更重要的是,FISH 对于未知的伙伴基因的检出显得无能为力. 2.2MLL 新...
Furthermore, AML samples known to have MLL-PTD can also be detected by analysis of the TCGA SNP-array database (Supplementary Figure 15e), suggesting that SNP-array is a valid platform for the detection of MLL-PTD. Upon further examination of 1000 different cancer cell lines in CCLE, 10 ...
1.2 13例MLL基因重排阳性AML中2例MLL/AF6,4例MLUAF9,1例 MLUAFl7、1例MLI/ELL,2例MLUAFl0,3例MLL.PTD。 1.3 13例MLL基因重排阳性AML中12例接受治疗,10例患者获得完全缓解 (complete remission,CR)。但总生存期较短。 1.4对8例AML患者定期监测相应的MLL基因重排,发现持续阴性者可长期存活, 阳性者则很快...
RT-PCR assay for detection of MLL-CT45A2 fusion transcripts was performed with a forward primer (MLL-S; 5'-GAGGATCCTGCCCCAAAGAAAAG-3') located in MLL exon 8 (GenBank accession no. NM_005933) and a reverse primer (CT45A2-AS; 5'- GGCCATCCTCTGCCTTTTC-3') located in CT45A2 exon ...
Adult ALL (n = 415) displayed 332 AF4, 9 AF9, 50 ENL, 1 AF10, 1 ELL, 1 MLL PTD, 9 AF6 and 4 EPS15 gene fusions. Additional 8 MLL rearrangements were identified (11q23, ACTN4, CEP164, KIF2A, MAML2, PRRC1, PTD and TET1). Adult AML (n = 373) displayed 3 AF4, 90 ...
最常见的11q23染色体易位为t(9;11) (p22;q23),形成MLL-AF9融合基因[6];其他常见的染色体易位还有dupMLL(11q23)、t(6;11) (q27;q23)、t(10;11) (p12;q23)、t(11;19) (q23;p13.1)及t(11;19) (q23;p13.3),分别形成MLL-PTD、MLL-AF6、MLL-AF10、MLL-ELL和MLL-ENL融合基因[7]。 MLL...
CONCLUSION: The combination of FISH and multiplex RT - PCR increases the sensitivity for detection of MLL rearrangement.%目的:采用荧光原位杂交(FISH)与逆转录多重巢式聚合酶链反应(multiplex RT-PCR)技术检测急性白血病中MLL基因重排的情况,分析两者联合应用的诊断价值.方法:对2008年1月～2011年5月在我院...
检测MLL基因重排类型包括MLL/AF4、MLL/AF6、MLL/AF9、MLL/AF10、MLL/AF17、MLL/ELL、MLL『E套Ⅱ、MLL/AFX、MLL/AFlP、MLL/AFlQ和MLL.PTD等;采用流式细胞技术检测AL的免疫表型;采用PCR法检测FLT3/ITD突变,阳性患者进行PCR产物测序。结果139例AL中检测到MLL基因重排10例,其中AML中MLL基因重排者7例,占7.3%(...