b第14天总白细胞(WBC)计数和c GFP阳性WBC计数。A1(d)和B1(e)细胞株的突变数目。 结果二:小鼠MLL/AML细胞突变的累积和小鼠MLL/AF9 AML候选致病突变。结果提示,在缩短小鼠MLL/AF9-AML白血病发病时间方面,获得驱动基因尤其是Gnb2的突变比突变积累本身更...
Percentage of GFP+ leukemia cells was higher in the spleen of moribund mice in the Arid1bfl/fl Mx1Cre cohort, compared with the control cohort. Taken together, our data suggests Arid1b, in addition to Arid2, acts as a potential novel tumor suppressor in MLL-AF9 leukemogenesis.\nTo study ...
MLL-AF9 is driven by the LTR promoter in the retroviral vector.29–31 LTR promoter activity can be silenced in ES cells mainly by KAP1 (KRAB-associated protein 1)- or Dnmts (DNA methyltransferases)-mediated methylation.32–34 As expected, GFP was not expressed in L-iPS cells (Figure 3a...
方法采用免疫磁珠法富集介导雌激素受体一重组酶Cre( ER.Cre) 的ADARl “““ 及其对照ADARl ”““ 小鼠骨髓Li neage一( Li n一) 细胞,用携带M SCV-M LL/AF9.IRES.GFP的逆转录病毒感染上述Li n一细胞,流式细胞... 文档格式:PDF | 页数:7 | 浏览次数:39 | 上传日期:2015-07-14 21:59:09 | ...
A leukemogenic MLL-AF9 cDNA was introduced into Mll−/− cells using a modified MSCV-based vector (Pear et al., 1998) that expresses GFP. Cells were harvested for quantitative RT-PCR and ChIP assays 2 weeks following transduction. Chromatin Immunoprecipitation (ChIP) and Standard PCR ...
为了分析t(4; 11)融合蛋白表达对H3K4和H3K79组蛋白甲基化的影响,使用MLL-AF4,AF4-MLL或AF4-MLL :: GFP在293T细胞中进行了瞬时转染实验,流式分析结果表明MLL-AF4和AF4-MLL具有H3K79me3 HMT活性,而AF4-MLL复合物还具有H3K4me3...
The MLL-AF9 oncogene is driven by the murine stem cell virus (MSCV) promoter, and the firefly luciferase was cloned under the elongation factor-1 alpha (EF1α) promoter, which has been demonstrated to express strongly in hematopoietic cells.17 GFP in traditional leukemogenic vectors was ...
图6为lamp5-as1调控mll-r白血病细胞自噬聚集。在构建有mrfp-gfp-lc3的mv4-11(a)和molm13细胞(b)中,敲低lamp5-as1后,动态监测细胞自噬情况。巴佛洛霉素a1(bafilomycina1)为自噬抑制剂。 图7为敲低lamp5-as1促进mll-af9的自噬降解。(a)在mll-r白血病细胞系中分别加入氯喹(chloroquine),巴佛洛霉素a1(bafilomycin...
本Trizol裂解液用于实验时,建议同时选购无任何靶向的对照细胞Trizol裂解液Control Knockout HEK293T Trizol Lysate (L00031)或靶向GFP的对照Trizol裂解液GFP Knockout HEK293T Trizol Lysate (L00033)。碧云天同时提供基于CRISPR/Cas9技术的MLLT3基因敲除的质粒(L28670 pLenti-MLLT3-sgRNA)、慢病毒(L28671 MLLT3 ...
The retroviral packaging cell line PA317 (selected in HAT medium) was transfected with linearised miR30- shRNA plasmid DNA (for both NS control and MLL198) using Lipofectamine, and GFP-positive cells were selected with puromycin. Stably transfected retro- viral-producing PA317 cell lines were ...