A total of 20–50 μg lysates were loaded onto SDS-PAGE gels and transferred to a PVDF membrane (Millipore, China). After blocking with TBS/T (0.1% Tween-20) containing 5% nonfat dry milk for 1 h at room temperature, the membranes were incubated with primary antibody at 4 °C over...
Cells were lysed on ice for 30 min in lysis buffer containing 50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1% Nonidet P-40, and 0.1% SDS supplemented with protease inhibitors (10 mg/ml leupeptin, 10 mg/ml pepstatin A, and 10 mg/ml aprotinin). Protein fractions were collected by ...
本发明涉及MEKK1‑MKK1/2‑MPK4级联对抗STOP1蛋白的磷酸化和稳定作用,具体地,本发明提供一种STOP1蛋白的磷酸化位点以及相应的磷酸化的STOP1蛋白,以及磷酸化的STOP1蛋白或其促进剂和/或MEKK1‑MKK1/2‑MPK4信号通路促进剂在提高植物的抗铝毒能力方面的用途。本发明首次发现,促进植物中STOP1蛋白的第386位...
(NaSH)、GYY4137、CompundC、STO-609、亚硝酸钠(NaNO2)、磷酸(Phosphoricacid,H3PO4,215104)、磺胺(Sulfamilamide,C6H8N2O2S,S9251)、N-(1-萘基)乙二胺二盐酸盐(Naphthylethylene,C12H14N2·2HCl,222488)、过硫酸铵(APS)、十二烷基磺酸钠(SDS)、脱脂奶粉、玉米油、多聚赖氨酸(poly-D-lysine,PLL):Sigma...