HCCC-9810, RBE and QBC-939 Cells (treated under different conditions for 48 h) were lysed by RIPA containing protease and phosphatase inhibitors, and the proteins were quantitatively analyzed using BCA kit based on SDS-PAGE gel electrophoresis. During western blotting, enhanced fluorescence substrates...
The samples were separated by SDS-PAGE and the protein was transferred to PVDF membrane (MilliporeSigma, Burlington, MA, USA). The following antibodies were used in western blotting, such as β-actin (Affinity, T0022, 1:3,000), PPARγ (Abcam, 209350, 1:1,000), SIRT1 (Cell Signaling ...
The samples were separated by SDS-PAGE and the protein was transferred to PVDF membrane (MilliporeSigma, Burlington, MA, USA). The following antibodies were used in western blotting, such as β-actin (Affinity, T0022, 1:3,000), PPARγ (Abcam, 209350, 1:1,000), SIRT1 (Cell Signaling ...
描述是一种有效的 MKK3 和 MKK6 抑制剂。 MDLMFCD00017680 别名棉子皮亭 英文名称Gossypetin CAS489-35-0 分子式C15H10O8 分子量318.24 储存条件-20℃ 纯度HPLC≥90% 外观(性状)Yellow Crystals 单位瓶 生物活性Gossypetin 是六羟基化的类黄酮,是一种有效的 MKK3 和 MKK6 抑制剂,可强烈减弱 MKK3/6-p38...
Equal amounts of lysates (20–50 μg) were loaded on a Mini-Protean TGX (4%–20%) SDS-PAGE (Bio-Rad). The separated proteins were transferred onto polyvinylidene difluoride membranes (Amersham). For phospho-specific antibodies, the membranes were blocked for 1 hr with blocking buffer ...
A total of 20–50 μg lysates were loaded onto SDS-PAGE gels and transferred to a PVDF membrane (Millipore, China). After blocking with TBS/T (0.1% Tween-20) containing 5% nonfat dry milk for 1 h at room temperature, the membranes were incubated with primary antibody at 4 °C over...
质粒提取试剂盒Plasmid Midi Prep购自Promega公司;真核细胞转染试剂Lopofectamin2000购自Invitrogen公司Aprotinin、PMSF以及Na3VO4均购自Sigma公司;SDS-PAGE系统及转印系统购自Bio-RAD公司;转引用硝酸纤维素膜Hybond-C购自Amersham公司;辣根酶底物的ECL购自GE公司;RACK1抗体购自BD Biosicences公司;MKK7抗体购自Cell Si...
(NaSH)、GYY4137、CompundC、STO-609、亚硝酸钠(NaNO2)、磷酸(Phosphoricacid,H3PO4,215104)、磺胺(Sulfamilamide,C6H8N2O2S,S9251)、N-(1-萘基)乙二胺二盐酸盐(Naphthylethylene,C12H14N2·2HCl,222488)、过硫酸铵(APS)、十二烷基磺酸钠(SDS)、脱脂奶粉、玉米油、多聚赖氨酸(poly-D-lysine,PLL):Sigma...
本发明涉及MEKK1‑MKK1/2‑MPK4级联对抗STOP1蛋白的磷酸化和稳定作用,具体地,本发明提供一种STOP1蛋白的磷酸化位点以及相应的磷酸化的STOP1蛋白,以及磷酸化的STOP1蛋白或其促进剂和/或MEKK1‑MKK1/2‑MPK4信号通路促进剂在提高植物的抗铝毒能力方面的用途。本发明首次发现,促进植物中STOP1蛋白的第386位...