We have characterized a subset of B lymphocytes expressing six- to eightfold higher levels of CD1 than the bulk of B cells. The cells have a surface phenotype (CD21(high), CD23(low), IgM(high), IgD(low)) found previously to characterize B cells residing in the splenic marginal zones. ...
The cell pellet was transferred to a fresh confluent monolayer of OP9-DL1 or OP9 cells in complete medium containing Flt3L and IL-7. Lymphoid cells grown in vitro were separated from OP9-DL1-EGFP/OP9-EGFP before performing any FACS analysis. Because cell population grown on either OP9 or...
63 The reaction was then rendered one-way by treating one of the cell populations with mitomycin C or X-irradiation that inhibits DNA synthesis, but does not impair the capability of these (stimulator) cells to induce proliferation of the other (responder) cell population.64 The MLR was the ...
Results: In the primary histologic areas of spongiotic dermatitis, a mixed population of B and T lymphocytes was seen. Ki-67 antigen proliferative index staining was accentuated in these areas, correlating with the presence of large numbers of epidermal and dermal antigen presenting cells. Among ...
In addition, in the same five patients, T cell response to PHA was inhibited by a cell in the non-T cell fraction, the response of non-T cells to PWM was diminished, and there was a diminished ability of the non-T cell population to stimulate in allogeneic MLR. However, the response...
To produce cultures in which one-way stimulation occurs the lymphocytes of one partner must be capable of responding to an antigenic stimulus, while the cells from the second partner should be immunologically unreactive and yet stimulatory. This second population can be produced in a number of ...
reported a significantly lower cumulative 10-year survival from the time of diagnosis in their series of 231 cryoglobulinemic patients age and sex-matched with individuals from the general population [41]. Other factors recognized as associated with a poor prognosis are renal involvement, widespread ...
(high active Rho), it remains unclear how they interact collectively to induce other modes of migration detected in Chinese hamster Ovary (CHO-K1) cells. We previously developed a six-variable (6V) reaction–diffusion model describing the interactions of these three proteins (in their active/...
(A) Adenosine is shown to skew DC differentiation and maturation toward a CD1a low CD14+ cell population, thereby inducing phenotypically dysregulated DCs. (B) While control mDC displays a MLR response – as expected, Adenosine strongly alters the allostimulatory ability of DCs to elicit T cell...
More than 20 years ago a striking observation was made in primary mixed lymphocyte cultures in vitro: When naive T cells from one inbred mouse strain were cultured with a genetically dissimilar, H-2-identical population of stimulator cells, up to 20% of all T cells were induced to proliferate...