As shown in part (Figure 4A), primer set BAMQ#8 would not amplify genomic DNAs from 20 different non- target species of commonly-encountered Gram positive and Gram negative bacteria tested at the concentration 10 million-fold higher than target DNA of A. cberi. The PCR primers BAMQ#8 ...
5000. A total of 250 µL of each sample were loaded in duplicate on a 96-well plate, and 2.5 µL of SYBR Green 100× was added to it. The 96-well plate was then wrapped in aluminum foil and placed for 20 min at 37 °C to allow the reaction to occur. A negative control ...