Onion Root Tip Lab- view real cells with a microscope, requires lab equipment and prepared slides Cancer: Out of Control Cells- article describing how the cell cycle relates to cancer, includes questions
Light microscopy For immunolabelled cells, we used poly-l-lysine-coated Ibidi chamber slides (eight-well, Ibidi 80826). The wells were filled with 1× PBS and 0.4 µM Hoechst 33342 (ThermoFischer 62249) was added. Immunostained cells were added to wells and allowed to settle for an ...
Protein Block was used to cover the slides for 10 min followed by incubation with primary antibodies overnight. The polymer was used for polymerization. The peroxidase activity was visualized with diaminobenzidine tetrahydroxychloride (DAB) solution. The sections were counterstained with hematoxylin (...
Slides were imaged using a Leica DMI6000 microscope and analysed using the MetaMorph software (Molecular Devices). Scoring polyploid cell population in human cells. Asynchronous growing cells were fixed with 70% ethanol, stained with PI, passed through 40 mm filter to remove aggregated cells. ...
The centrosome is the master orchestrator of mitotic spindle formation and chromosome segregation in animal cells. Centrosome abnormalities are frequently observed in cancer, but little is known of their origin and about pathways affecting centrosome homeostasis. Here we show that autophagy preserves centro...
Slides were counterstained with 4′,6 diamidino-2-phenylindole (DAPI) and phalloidin and were mounted in ProLong gold before visualization. Confocal microscopy was done on an inverted Leica SP8 3 channel confocal system at ×63 magnification. Image analysis was performed with Fiji software (National...
Protein Block was used to cover the slides for 10 min followed by incubation with primary antibodies overnight. The polymer was used for polymerization. The peroxidase activity was visualized with diaminobenzidine tetrahydroxychloride (DAB) solution. The sections were counterstained with hematoxylin (...
A containing 0.15 M NaCl, 0.02 M Tris, 0.05% Tween 20, pH 7.4 for 5 min. Plus and minus PLA probes (secondary antibody conjugated to a unique oligonucleotide; plus for rabbit and minus for mouse) were added to the slide and incubated for 1 h. After incubation, the slides...
Immunolabeling was conducted on Bouin-fixed and paraffin-embedded testicular sections, which were mounted on silane-treated glass slides (Histobond Superior; Paul Marienfeld Laboratory Glassware, Laud-Königshofen, Germany) and dried at 37 °C for 24 h. In order to confirm successful Cx43 gene ...
The slides were mounted with DAPI (Invitrogen). The images were captured using a Zeiss 710 confocal laser scanning microscope (Carl Zeiss, Jena, Germany). 2.9. RNA Isolation, Reverse Transcription, and RT-PCR RNA was extracted [47] and reverse transcribed into cDNA for RT-PCR, as previously...