Kondrat’eva, E. V.Bull Exp Biol MedBulletin of Experimental Biology & MedicineLobanova EG, Kor drat' eva EV. Measurement of mi- tochondrial membrane potential in leukocyte suspen- sion by fluorescent spectroscopy[J]. Bull Exp Biol Med, 2014,157 (2) : 288-290....
青云英语翻译 请在下面的文本框内输入文字,然后点击开始翻译按钮进行翻译,如果您看不到结果,请重新翻译!Measurement of mitochondrial membrane potential选择语言:从 到 翻译结果1翻译结果2 翻译结果3翻译结果4翻译结果5 翻译结果1复制译文编辑译文朗读译文返回顶部 线粒体膜电位的测量 翻译结果2复制译文编辑译文朗读...
3.5Mitochondrial membrane potential measurement Themitochondrial membranepotential (ΔΨm) generated byproton pumps(complexes I, III and IV) is a key indicator of mitochondrial activity. It reflects the process ofelectron transportandoxidative phosphorylation. Theelectrochemical gradientacross theIMMcouples ATP...
Membrane potential measurement Membrane potential was determined by live confocal microscopy. HL-1 cardiac myocytes (10,000 cells/well) were plated in 96well CellCarrier Ultra imaging plates (PerkinElmer) coated with gelatin/fibronectin. The day of the experiment, nuclei were labeled with NucBlue Live...
Mitochondrial membrane potential measurement For measurement of mitochondrial membrane potential, far-red fluorescent indicator DilC1(5) (M34151, ThermoFisher) was used, following manufacturer instructions. In brief, cells transfected with vectors pGal-Trans-eGFP, pER-FlucWT-eGFP or pER-FlucDM-eGFP ...
2.4. Mitochondrial membrane potential measurement The mitochondrial membrane potential (ΔΨm) was indirectly monitored using the fluorescent probe 2-[4(dimethylamino) styryl]-1-methylpyridinium iodide (DASPMI) (Molecular Probes). Yeast samples grown in SD medium up to the exponential phase (0.3–0.6...
The electrode exhibits very good selectivity for TPP+ ion against the substances used for the measurement of membrane potential of mitochondria. The electrode was applied satisfactorily to the estimation of mitochondria1 membrane potential.doi:10.1080/00032719108052905...
Measurement of mitochondrial membrane fluidity Mitochondria were first isolated using mitochondria isolation kit (Beyotime, Shanghai, China). Mitochondria membrane fluidity was measured by monitoring the fluorescence polarization (P) of trimethylammonium 1,6-dipheny-1,3,5-hexatriene dye (TMA-DPH, Medche...
The initial applications of fluorescent lipophilic cations were to bulk measurement of the average membrane potential of cells or organelles in suspension; the dye concentrations used were sufficiently high that the fluorescence of most the dye molecules in the interior was quenched by virtue of the ...
Measurement of mitochondrial membrane potential Washed mouse platelets (1 × 108 ml−1) in PWB were incubated with TMRM (0.5 μM) for 15 min at 37 °C. Excess TMRM was then removed by centrifugation and platelets resuspended in Tyrode’s buffer. Samples were incubated with a com...