Mitochondria contain many copies of a circular DNA molecule (mtDNA), which has been observed as a mixture of normal and mutated states known as heteroplasmy. Elevated heteroplasmy at a single mtDNA site, m.3243A > G, leads to neurologic, sensory
Treating healthy wild-type primary neurons with damaged mtDNA from Ifnb−/− neurons (KOmtDNA) induced neuronal cell-death, while nuDNA at the same concentration did not have the same effect (Fig. 3A). Similar results were observed in N2A cells (Fig. S3A). Remarkably, only KO damaged...
The consequences of damage to the mitochondrial genome (mtDNA) are poorly understood, although mtDNA is more susceptible to damage resulting from some genotoxicants than nuclear DNA (nucDNA), and many environmental toxicants target the mitochondria. Reports from the toxicological literature suggest that...
Terms to be familiar with before you start to solve the test:mitochondrial DNA,oxidative phosphorylation,mtDNA mutations,homoplasmy,heteroplasmy,restriction endonucleases,mitochondrial protein translocation,DNA cloning,expression plasmid,recombinant DNA,transient transfection,immunocytochemistry,stable transfection,poly...
Mitochondrial DNA introgression conforms to the null demographic model Overall, our results are compatible with the invasion-replacement hypothesis and the nuclear and mitochondrial genomes share similar patterns of increased introgression towards the north. However, levels of nuclear introgression are much ...
Soto, et al., Results of the 1999-2000 collaborative exercise and proficiency testing program on mitochondrial DNA of the GEP-ISFG: an inter-laboratory study of the observed variability in the heteroplasmy level of hair from the same donor, Forensic Sci. Int. 125 (2002) 1- 7....
Comparison of atp1 cDNA and DNA sequences in Rafflesia cantleyi. A. Agarose gel showing 1: RT-PCR results obtained using an initial reverse transcription step during thermalcycling, 2: RT-PCR results obtained without an initial reverse transcription step during thermalcycling, 3: RT-PCR results...
[39]. Different results in the abundance of mtDNA copy numbers could be associated with different bioenergetic requirements of tumors or due to specific nuclear DNA or mtDNA mutation. For example, mtDNA content is significantly upregulated in endometrial carcinomas withTP53mutations or in low-grade ...
nuclear DNA integrity (Figs2AandS1C) but, at this early time point, did not affect mtDNA content (Fig.2B). We calculated that 0.003, 0.006 or 0.009 U/ml of GOx results in 0.27 ± 0.025, 1.75 ± 0.14 and 4.23 ± 0.84 DNA lesions/10 kb of mtDNA, respectively. ...
Results Individuals born after ART display a different mitochondrial DNA variant genotype than their spontaneously conceived peers First, we sequenced the full mitochondrial genome of 270 ART and 181 SC children (details on the participants and the source of DNA can be found in Table1). During dat...