Mitochondria were labelled with the mitochondria-targeted mScarlet protein (Mito). j, Western blot showing the expression of mutant P5CS in MEFs. The presence of the Flag tag confirms the expression of the mutant protein. Vinculin is used as a loading control. k, Representative live-cell ...
e, Relative abundance of mitochondrial M + 3 lactate in CD8+ Teff cells treated with 13C-labeled LA and/or LC/sotrastaurin. n = 8. f, Schematic sequence of the N terminus (NT) of MCT1 and diagram of targeting peptide–EGFP fusion protein. g, Colocalization of targeting ...
Mitochondria are labelled in pink. Scale bar = 2 lm; (bottom left and middle) Quantification of mitochondrial volume fraction (%Vv) and mitochondrial number per cross-section in proliferating and senescent (3 days after 20-Gy X-ray) MRC5 fibroblasts treated with or without ...
Following extensive washing, the membranes were incubated with an appropriate HRP-labelled secondary antibody. The blots were then developed using the enhanced Western Bright ECL reagent (Advansta, Menlo Park, CA, United States). The levels of protein expression were semi-quantified by optical ...
Figure 7. Fluorescence microscopic images (A) Rhodamine B labeled-PC/5d-liposomes (Nikon eclipse Ci, ×400), (B) curcumin labeled SLN/5d (Nikon eclipse Ci, ×1000) after incubation time (t = 0, 1, 6 and 24 h), cellular uptake study (flow cytometry) (A) Rhodamine B labelled-PC/5d...
Thus, Figure 2a outlines a graph with 4 nodes which will be transformed in new graph that provided the detail on the right part of the same image. As we can see, the nodes of this new graph are labelled by 𝑥𝑖xi and its edges are generated by 𝑞𝑖,𝑗qi,j, that formed ...