Green fluorescent protein (GFP) tags can also be used to visualize proteins in living cells, and this has been a useful tool in research on mitochondria dynamics. Plants perform two vital functions: photosynthesis, the fixation of solar energy to chemical energy in the form of glucose, which ...
A549 cells were co-transduced with CellLight® Late Endosomes-GFP CellLight® Golgi-RFP and incubated overnight. Following staining with MitoTracker® Deep Red, cells were imaged on a Zeiss LSM confocal microscope. Mitochondria of live bovine pulmonary artery endothelial cells stained with the ...
Further studies using the split-GFP system in human RPE1 cells and in yeast revealed that cytosolic proteins prone to aggregation are imported into mitochondria in order to undergo degradation by mitochondrial proteases, such as Pim129. This indicates that mitochondria play a role in both ...
full-length PINK-1/PINK1 (Ppink-1::PINK-1/PINK1::GFP) to identify a panel of small molecules that increase the level of PINK-1 protein (as monitored by the increased fluorescence of PINK-1::GFP fusion) and verified their effect on the activation of mitophagy and ...
Stably transformed Arabidopsis lines have been generated with GFP targeted to the mitochondria using either of two plant mitochondrial signal sequences from the beta-ATPase subunit or the mitochondrial chaperonin CPN-60. Mitochondrially targeted GFP, which is easily detectable using an epifluorescent or...
The first GFP form used, GFP1, is easily observable in cells with low autofluorescence, such as suspension cells or trichomes, but masked in green tissue. For the visualization of GFP in cells and tissues with high autofluorescence, such as leaf, the use of a very strong promoter (35S35S...
Green fluorescent protein (GFP) tags can also be used to visualize proteins in living cells, and this has been a useful tool in research on mitochondria dynamics. Plants perform two vital functions: photosynthesis, the fixation of solar energy to chemical energy in the form of glucose, which ...
PA-GFP 504 517 0.79 poor photo-activatable; can be modulated to target mitochondria Mito-RFP 555 584 0.48 medium monomeric; fast maturation; high pH stability PAFPs are, in principle, eligible for PALM or FPALM. However, for single-color PALM or FPALM imaging, PAFPs are not favored becaus...
中文名称: PT7-MTGFP(MITOCHONDRIA) 中文同义词: PT7-MTGFP(MITOCHONDRIA) 英文名称: pT7-mtGFP(mitochondria) 英文同义词: CAS号: 分子式: 分子量: 0 EINECS号: 信息错误报告 您的Email: 按国家浏览pT7-mtGFP(mitochondria)国外供应商 中国供应商 pT7-mtGFP(mitochondria) 推荐供应商 建议您优先...
In contrast, TAT-GFP, without a mitochondrial signal sequence, rapidly transduced into cells and mitochondria, displayed pseudo-first-order kinetics, but did not remain there. Mice injected 5 days prior with TAT-mMDH-eGFP had detectable eGFP activity in multiple tissue types. Western blotting of ...