(C) Mito-GFP-expressing atg32Δ cells were transformed with a low-copy plasmid that encodes no protein (p-empty), wild-type Atg32 (p-WT), or Atg32AQAA (p-AQAA) with the ATG32 5′ and 3′ UTRs, grown in glycerol medium, and observed using fluorescence microscopy at the indicated ...
33]. A database for nuclear proteins and their subnuclear location has been constructed[34]. The prediction of protein subcellular location has been extended to a new level, the subnuclear level[35,36], where the protein location within cell nucleus can be predicted...