This protocol describes adenoviral transduction, live-cell imaging, and quantification of mitochondrial oxidative status in primary culture of human coronary artery endothelial cells (HCAEC), using a mitochondrial-targeted and redox sensitive fluorescent marker known as mito-roGFP. The protocol requires the...
ECV304 cells lines constitutively expressing the cytoplasmic (cyto-roGFP) and mitochondrial (mito-roGFP) form of roGFP.Annalisa, CossuAnna, Maria PosadinoRoberta, GiordoCostanza, EmanueliAnna, Maria SanguinettiAmalia, PiscopoMarco, PoianaGiampiero, Capobianco...