HH-CAS-204 PB-rtTA-BsmbI (Plasmid #126028) HH-CAS-203 PB-TRE-Cas9 (Plasmid #126029) HH-CAS-202 DD-dSpyCas9-mCherry-APEX2(Plasmid #108570) HH-CAS-201 LentiCRISPRv2 GFP(Plasmid #82416) HH-Y-077 pET-28b-Cas9-His(Plasmid #47327) HH-CAS-200 Lenti-iCas9-EGFP-rtTA-Advanced(pindu...
P1225/mito-PAGFP线粒体定位质粒 产品简介:质粒(plasmid)是细菌或细胞染色质以外的,能自主复制的,与细菌或细胞共生的遗传成,质粒载体是在天然质粒的基础上为适应实验室操作而进行人工构建的质粒。与天然质粒相比,质粒载体通常带有一个或一个以上的选择性标记基因(如抗生素抗性基因)和一个人工合成的含有多个限制性...
pLVX-shFLCN recombinant plasmid map ;B :1% agarose gel electrophoresis detection map(M :DNA Marker ;1 :FLCN gene fragment digested by EcoR I /Xho I,1 740 bp ;2 :pLVX-shRNA vector fragment digested by EcoR I /Xho I,7 881 bp ;3 :pLVX-shFLCN plasmid DNA,9621 bp);C :FLCN gene ...
HH-Z-024 pCXWB-EBNA1(Plasmid #37624) 协助哺乳动物细胞中质粒复制,增强外源质粒转染效率和表达 HH-Z-102 pCMV6-AC-IRES-GFP 哺乳动物表达载体C-terminal Myc-DDK tag HH-Z-101 pTriEx-GST 真核-原核-昆虫三表达载体质粒 HH-Z-095 pEBFP-C1瞬转荧光蛋白质粒;蓝色荧光蛋白报告系统 HH-Z-096 pEYFP-Mem...
1b–d). To further support that the AP2 proteins are inside the mitochondrion, we employed the split-GFP complementation technique28. The plasmid encoding GFP1-10 fused to the mitochondrial matrix targeting sequence (MTS) of HSP60 (MTS-HSP60-GFP1-10) was introduced into 7xGFP11 tagged AP...
2.质粒(Plasmid)的概念 质粒是生物细胞内固有的、能独立于寄主染色体而自主复制、并被稳定遗传的一类核酸分子。质粒常见于原核细菌和真 菌中,绝大多数的质粒是 DNA 型的,少部分为 RNA 型。天然 DNA 质粒大都具有共价、封闭、环状的分子结构,其分 子量范围:1-300 kb。细菌质粒是基因工程中最常用的载体。 质粒...
GFP (horizontal stripes), in the pT2KXIGΔin plasmid containing the Tol 2 transposable element (gray). The binding of NF-Y to the CCAT boxes is shown. (B) Relative luciferase activity obtained in one to four-cell stage AB embryos transiently injected with the pT2KXIGΔin-Luc/GFP or ...
were introduced using NotI and PacI sites in the multiple cloning site. The plasmid for expression of single guide RNA andStreptococcuspyogenesCas9 (variant eSpCas9(1.1)) was derived from Addgene plasmid #71814, a gift from F. Zhang50. The 20-bp single guide RNA target sequence (5′-GTTTC...
The nucleases described above were cloned into a plasmid vector containing a T7 promoter, 5' and 3' untranslated regions and a polyT repeat to serve as a template for a >100 bp polyA tail. The DNA template was linearized immediately following the polyT tail, and the product purified using...
(QPCR) and Western blot.Results Sequencing results showed that the inserted sequence in the over-expression plasmid was consistent with the mouse OGG1 (NM_010957.4) gene sequence in the gene library.The original lentiviral titer after packaging and purification was between 2.0× 107to 6.0× 1...