Following transfection with the miRNA-21 inhibitor, SKOV3/DDP cells demonstrated decreased sensitivity to cisplatin compared with negative control cells. Furthermore, PTEN mRNA expression levels in SKOV3 cells transfected with miRNA-21 mimics was significantly lower compared with negative control cells. ...
[0002] 微小RNA-21 (microRNA-21,miR-21)是miRNA家族中的成员之一,具有重要的生物 学功能。目前,通过改变miR-21的活性或表达,从而研究其生物学功能的方法主要有抑制 剂(Inhibitor)、拮抗剂(Antagomir)及慢病毒载体等。然而,这些方法存在有效作用时间 短、操作困难、安全性能差等缺点,难以达到长效、便捷下调miR-21...
miRNA-21在糖尿病肾病中的作用机制及 研究进展 刘 静1，张 鹏2* 1 西安医学院，陕西 西安 2第四军医大学西京医院肾内科，陕西 西安 收稿日期：2021年11月27日；录用日期：2021年12月17日；发布日期：2021年12月30日 摘 要 糖尿病肾病(DKD)是糖尿病引起的严重微血管并发症，其发病机制复杂且临床上...
(90.180.270nmo1/L)ofmiRNA一21inhibitorandweretransfectedbothce11sanda negativecontr01weretransfectedamixtureofLipofectamine。M2000reagent,respectively.AfteranOther 3一davcultIlre,tllepro1ifemtionofcellswasdetectedbvceUcountingkit一8,andRvaluewasca1culatedto ...
6b). Moreover, the activation of HSCs was abolished by miRNA-21 inhibitor and AKT inhibitor (Fig. 6b). To further examine whether cancer cell-derived exosomes were associated with lipogenesis in HSCs, we assessed the severity of steatosis by oil eed assay. Compared with control group and LO2...
Macrophage-secreted Exosomes Delivering miRNA-21 Inhibitor can Regulate BGC-823 Cell Proliferation 来自 Semantic Scholar 喜欢 0 阅读量: 197 作者:JJ Wang,ZY Wang,C Rui,X Jing,YL Yao 摘要: Exosomes, membranous nanovesicles, naturally carry bio-macromolecules or miRNA and play impoetant roles in ...
Cys C is a cysteine protease inhibitor, and its half-life is only 1.5 h (for comparison, Scr’s half-life is 4 h)43. The level of Cys C only depends on the glomerular filtration ability, and experiments have shown that the biological change in Cys C is only 7% of that in Scr...
miR-21inhibitorandupregulatedbypre-miR-21.H 2 O 2 -inducedcardiaccelldeathandapoptosiswere increasedbymiR-21inhibitorandwasdecreasedbypre-miR-21.Programmedcelldeath4(PDCD4)that wasregulatedbymiR-21andwasadirecttargetofmiR-21incardiacmyocytes.Pre-miR-21-mediated ...
When miR-21 and Sox2 were suppressed, a β-catenin agonist compound could restore the invasion potential of glioma cells, while a β-catenin signaling inhibitor significantly hindered the miR-21/Sox2-induced invasion potential, showing that the miR-21/Sox2/β-catenin axis promotes glioma ...
miR-21 regulates a number of processes and pathways relevant to tumorigenesis including PDCD4, a regulator of apoptosis; PTEN, a suppressor of PI3K/AKT signaling; Smad7 and MSH2, regulators of TGFβ signaling; and Sprouty 1, an inhibitor of ERK-MAPK signaling. Here we describe an additional...